Antioksidacijska svojstva odabranih Micromeria vrsta

master's thesis

• Title (english): Antioxidative properties of selected micromeria speies
• Author: Monika Švob
• Mentor: Maja Bival Štefan (mentor)
• Committee member: Maja Bival Štefan (predsjednik povjerenstva)
• Committee member: Biljana Blažeković (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2021-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada provedena su dva spektrofotometrijska istraživanja tri odabrane
Micromeria vrste hrvatske flore – M. croatica, M. thymifolia i M. juliana. Prvo spektrofotometrijsko
istraživanje koje smo proveli određivanje je sposobnosti hvatanja slobodnih radikala dušikovog (II)
oksida u usporedbi s poredbenim otopinama aksorbinske i ružmarinske kiseline, te rutina. Sve tri
Micromeria vrste pokazale su antioksidacijski potencijal (IC50 81,5-88,1 μg/ml), dok je M. croatica
pri najnižoj koncentraciji (81,5 μg/ml) neutralizirala 50% slobodnih radikala. M. thymifolia ipak je
neutralizirala preko 70% slobodnih radikala pri većim koncentracijama (400-1600 ug/ml).
Drugo spektrofotometrijsko istraživanje koje smo proveli određivanje je sposobnosti inhibicije
lipidne peroksidacije u usporedbi s poredbenim otopinoma rutina i ružmarinske kiseline. Sve tri
Micromeria vrste pokazale su sposobnost inhibicije (IC50 54,5-206 μg/ml). Najveću antioksidacijsku
sposobnost imala je M. croatica koja je već pri koncentraciji od 54,5 μg/ml neutralizirala 50%
slobodnih radikala.

Abstract (english)

Two spectrophotometrical researches of three Micromeria species of Croatian flora – M. croatica,
M. thymifolia and M. juliana where examined in this thesis. First spectrophotometrical research we
examined was capacity of inhibition of free NO(II) radicals compared to ascorbic acid, rosmarinic
acid and rutin. All three Micromeria species expressed antioxidative capacity (IC50 81,5 – 88,1
μg/ml), while M. croatica had neutralized 50% of free radicals at lowest concentration (81,5 μg/ml).
M. thymifolia had neutralized over 70% of free radicals at highest levels of concentration (400-1600
μg/ml).
Second spectrophotometrical research we conducted was to determine capacity of inhibition of lipid
peroxidation in comparison to rutin and rosmarinic acid. All three Micromeria species expressed
antioxidative capacity (IC50 54,5-206 μg/ml). M. croatia had highest capacity of three examined
species and had neutralized over 50% of free at concentration of 54,5 μg/ml.

• Keywords: dušikov (II) oksid; lipina peroksidacija; Micromeria vrste
• Keywords (english): nitric oxide; lipid peroxydation; Micromeria species
• Language: croatian
• URN:NBN: urn:nbn:hr:163:708841
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-08 14:32:54