Usporedba klasične i instrumentalne analitičke metode za određivanje sadržaja kalcija u uzorcima dodataka prehrani

master's thesis

• Title (english): Comparison of classical and instrumental analytical methods for determination of calcium content in samples of food supplements
• Author: Sanja Ursula Herega
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Marijana Zovko Končić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2021-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract

Kalcij je esencijalni makronutrijent važan za normalno funkcioniranje mnogih procesa u ljudskom organizmu. Nedostatak kalcija u organizmu nadomješta se dodacima prehrani.
Kao uzorci za usporedbu dviju analitičkih metoda i određivanje sadržaja kalcija poslužili su dodaci prehrani dvaju različitih proizvođača.
Cilj ovog diplomskog rada bio je usporediti klasičnu – gravimetrijsku i instrumentalnu – FAAS metodu u svrhu određivanja sadržaja kalcija u dodacima prehrani. Obje metode su visoke točnosti i preciznosti. Provedenim istraživanjem uočene su prednosti određivanja koncentracije kalcija plamenom tehnikom koja se pokazala kao jednostavnija i brža metoda naročito za veći broj uzoraka. Niske dobivene RSD vrijednosti upućuju na zadovoljavajuću ponovljivost određivanja koncentracije kalcija plamenom tehnikom. Na temelju dobivenih vrijednosti LOD i LOQ te vrijednosti za točnost provedene metode, plamena tehnika se može smatrati prikladnom za određivanje kalcija u uzorcima dodataka prehrani te u usporedbi s klasičnom gravimetrijskom metodom i metodom izbora.

Abstract (english)

Calcium is an essential macronutrient that is important for the normal functioning of many processes in the human body. A deficiency of calcium in the body is compensated for dietary supplements.
Dietary supplements from two different manufacturers served as samples to compare the two methods of analysis and determine the calcium content.
The aim of this work was to compare the classical - gravimetric and the instrumental - FAAS method for the determination of calcium content in food supplements. Both methods are characterised by high accuracy and precision. The study showed the advantages of the determination of calcium concentration by FAAS, which proved to be an easier and faster method, especially with a larger number of samples. The obtained low RSD values indicate a satisfactory repeatability of the determination of calcium concentration by the flame technique. Based on the obtained LOD and LOQ values and the values for the accuracy of the performed method, FAAS can be considered suitable for the determination of calcium in samples of food supplements and the method of choice compared to the classical gravimetric method.

• Keywords: gravimetrija; atomska apsorpcijska spektrofotometrija; kalcij; dodaci prehrani; validacija
• Keywords (english): gravimetric; atomic absorption spectrometry; calcium; dietary supplements; validation
• Language: croatian
• URN:NBN: urn:nbn:hr:163:431173
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-03-29 09:47:41