Uloga izvanstaničnih adenozin-trifosfata i proteina toplinskoga šoka 70 u kroničnoj opstrukcijskoj plućnoj bolesti

master's thesis

• Title (english): Role of extracellular adenosine-triphosphate and heat shock protein 70 in chronic obstructive pulmonary disease
• Author: Matea Tomljanović
• Mentor: Lada Rumora (mentor)
• Committee member: Lada Rumora (predsjednik povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2022-03-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Kronična opstrukcijska plućna bolest je treći vodeći uzrok smrtnosti u svijetu i time predstavlja veliki izazov moderne
medicine. Svjetska zdravstvena organizacija pretpostavlja značajan porast broja oboljelih u budućnosti zbog povećane
prisutnosti rizičnih čimbenika, od kojih su najznačajniji aktivno i pasivno pušenje te onečišćenje zraka. Unatoč velikoj
prevalenciji, dijagnostika i liječenje su još uvijek nedostatni. Velik broj oboljelih nema postavljenu dijagnozu, dijelom
zbog nedovoljne edukacije samog pacijenta koji simptome ne prepoznaje, a dijelom zbog nedovoljnih dijagnostičkih
metoda. Liječenje može smanjiti simptome i spriječiti buduće egzacerbacije, ali ne može dovesti do izlječenja.
Pacijenti koji boluju od KOPB-a imaju značajno narušenu kvalitetu života, a troškovi liječenja predstavljaju problem i
pacijentima i zdravstvenom sustavu. Kako bi se omogućila pravovremena dijagnostika i postigli bolji ishodi liječenja,
potrebno je identificirati i razjasniti patofiziološke mehanizme bolesti koji su trenutno kompleksni i nepoznati.
Cilj ovog diplomskog rada je napraviti pregled dostupnih podataka o KOPB-u te izvanstaničnom ATP-u i
izvanstaničnom Hsp70 kao potencijalnim biomarkerima. eATP i eHsp70 su molekule koje, kad se nalaze izvan
stanice, djeluju kao molekulski obrasci povezani s oštećenjem. Imaju važnu ulogu u upalnim procesima kod različitih
bolesti, pa tako i KOPB-a, a dosadašnja istraživanja potvrđuju njihovu povezanost s KOPB-om. Zabilježene su njihove
povećane koncentracije u pacijenata s KOPB-om u usporedbi sa zdravim ispitanicima. Daljnja istraživanja mogla bi
potvrditi njihovu ulogu kao potencijalnih biomarkera koji omogućuju raniju dijagnozu bolesti i olakšavaju
klasifikaciju pacijenata prema GOLD smjernicama. Saznanja o njihovom točnom djelovanju i signalnim putovima
mogla bi dovesti do razvoja novih dijagnostičkih metoda ili otkrivanja novih terapijskih meta, a time i do uspješnijeg
liječenja i smanjenja mortaliteta.

Abstract (english)

Chronic obstructive pulmonary disease is the third leading cause of death in the world and thus represents a major
challenge for modern medicine. The World Health Organization assumes a significant increase in the number of patients
in the future due to the increased presence of risk factors, the most significant of which are active and passive smoking
and air pollution. Despite the high prevalence, diagnosis and treatment are still insufficient. A large number of patients
have not been diagnosed, partly due to insufficient education of the patients who do not recognize the symptoms, and
partly due to insufficient diagnostic methods. Treatment can reduce symptoms and prevent future exacerbations, but it
cannot cure the disease. Patients suffering from COPD have a significantly impaired quality of life, and the cost of
treatment is a problem for both patients and the health care system. In order to enable timely diagnosis and achieve better
treatment outcomes, it is necessary to identify and clarify the pathophysiological mechanisms of the disease that are
currently complex and unknown.
The aim of this thesis is to review the available data on COPD and extracellular ATP as well as extracellular Hsp70 as
potential biomarkers. eATP and eHsp70 are molecules that, when present outside the cell, act as danger-associated
molecular patterns. They play an important role in inflammatory processes in various diseases, including COPD, and
previous research confirms their association with COPD. Increased concentrations have been reported in patients with
COPD compared to healthy subjects. Further research could confirm their role as potential biomarkers that enable earlier
diagnosis of the disease and facilitate the classification of patients according to the GOLD guidelines. Knowledge of
their exact action and signalling pathways could lead to the development of new diagnostic methods or the discovery of
new therapeutic targets, and thus to more successful treatment and reduced mortality.

• Keywords: kronična opstrukcijska plućna bolest; KOPB; ATP; Hsp70
• Keywords (english): chronic obstructive pulmonary disease; COPD; ATP; Hsp70
• Language: croatian
• URN:NBN: urn:nbn:hr:163:043621
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-03-31 12:29:51