Antioksidacijski status pacijentica suplementiranih α-lipoičnom kiselinom s dijagnozom cervikalne intraepitelne neoplazije stupnja 1 i 2

master's thesis

• Title (english): Antioxidant status of α-lipoic acid supplemented patients diagnosed with grade 1 and 2 cervical intraepithelial neoplasia
• Author: Marta Ivanko
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-05-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Antioksidativni status organizma ovisi o brojnim čimbenicima životnog stila kao što su indeks tjelesne mase
(ITM), pušenje, karakteristike prehrane, uzimanje dodataka prehrani itd. U okviru ovog istraživanja ispitan
je utjecaj navedenih karakteristika životnog stila i suplementacije α-lipoičnom kiselinom (ALA) (600 mg/dne;
3 mjeseca), na antioksidacijski status 100 pacijentica sa utvrđenom dijagnozom cervikalne intraepitelne
neoplazije stupanj 1 i 2 (CIN1 i CIN2). Antioksidacijski status utvrđen je praćenjem promjene koncentracije
reduciranog glutationa (GSH) u krvnom serumu pacijentica. Utjecaj suplementacije ALA na koncentracije
GSH u serumu istražen je u okviru dvostruko-slijepe randomizirane placebo-kontrolirane studije korištenjem
standardne fluorimetrijske metode, a utjecaj prehrane i karakteristika životnog stila korištenjem validiranog
upitnika o učestalosti konzumacije namirnica. Rezultati ovog istraživanja pokazali su kako ITM, pušenje te
uzimanje dodataka prehrani s antioksidansima nemaju bitan utjecaj na antioksidacijski status pacijentica.
Također, suplementacija α-lipoičnom kiselinom u dozi od 600 mg dnevno kroz 90 dana nije pokazala
značajan utjecaj na antioksidacijski status pacijentica s dijagnozom CIN1 i CIN2. Nasuprot tome, pacijentice
s visokim unosom povrća imale su značajno više koncentracije GSH u serumu. Kako bi se dobili što
relevantniji rezultati i bolji uvid u antioksidacijsku sposobnost α-lipoične kiseline potrebno je provesti daljnja
istraživanja koja uključuju veći broj ispitanica, kao i osjetljivije biomarkere antioksidacijskog statusa ili
kombinaciju istih.

Abstract (english)

The antioxidant status of the organism depends on a number of lifestyle factors such as body mass index
(BMI), smoking, dietary characteristics, taking dietary supplements, etc. The study examined the impact of
these lifestyle characteristics and α-lipoic acid (ALA) supplementation characteristics (600 mg / day; 3
months) on the antioxidant status of 100 patients diagnosed with grade 1 and 2 cervical intraepithelial
neoplasia (CIN1 and CIN2). Antioxidant status was determined by monitoring changes in reduced glutathione
(GSH) levels in the blood serum of patients. The effect of ALA supplementation on serum GSH
concentrations was investigated in a double-blind randomized placebo-controlled study using a standard
fluorimetric method, and the effect of diet and lifestyle characteristics using a validated food consumption
frequency questionnaire. The results of this study showed that BMI, smoking and taking dietary supplements
with antioxidants do not have a significant impact on the antioxidant status of patients. Also, α-lipoic acid
supplementation at a dose of 600 mg daily for 90 days did not show a significant effect on the antioxidant
status of patients diagnosed with CIN1 and CIN2. On the contrary, patients with high vegetable intake had
significantly higher serum GSH concentrations. In order to obtain the most relevant results and better insight
into the antioxidant capacity of α-lipoic acid, it is necessary to conduct further research involving a larger
number of subjects, as well as more sensitive biomarkers of antioxidant status or a combination thereof.

• Keywords: oksidacijski stres; antioksidans; glutation; α-lipoična kiselina; cervikalna intraepitelna neoplazija
• Keywords (english): oxidative stress; antioxidant; glutathione; α-lipoic acid; cervical intraepithelial neoplasia
• Language: croatian
• URN:NBN: urn:nbn:hr:163:917619
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-05-02
• Created on: 2022-05-25 13:47:44