Ispitivanje prisutnosti sterigmatocistina u pivu metodom tankoslojne kromatografije

master's thesis

• Title (english): Investigation of the presence of sterigmatocystin in beer by thin-layer chromatography
• Author: Ana Šmehil
• Mentor: Daniela Jakšić (mentor)
• Committee member: Daniela Jakšić (predsjednik povjerenstva)
• Committee member: Maja Šegvić Klarić (član povjerenstva)
• Committee member: Ana-Marija Domijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2022-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Sterigmatocistin (STC) je citotoksični i genotoksični mikotoksin kojeg dominantno proizvode
plijesni roda Aspergillus iz serije Versicolores. Ove su plijesni široko rasprostranjene u prirodi, ali i
unutar zatvorenih prostora, u koje spadaju i oni u kojima se skladišti hrana i sirovine za njenu
proizvodnju, što upućuje na mogućnost kontaminacije te hrane i sirovina ovim mikotoksinom.
Kontaminacija STC-om do sada je utvrđena u žitaricama, zrnima kave, začinima, orašastim
plodovima, u hrani za životinje te u siru, dok o pojavnosti ovog mikotoksina u pivu nema mnogo
literaturnih podataka. S obzirom na visoku potrošnju ovog popularnog pića u Hrvatskoj i u svijetu
može se pretpostaviti nepovoljan učinak na zdravlje izloženih ljudi uslijed izloženosti ovom
mikotoksinu. Kako bi se detektirala pojavnost STC-a u pivu prikupljeno je 58 uzoraka piva s
hrvatskog tržišta. Ekstrakcija je provedena kombiniranjem ekstrakcije acetonitrilom uz isoljavanje
te pročišćavanje pomoću SPE kolona. Tako pripremljeni ekstrakti analizirani su metodom
tankoslojne kromatografije uz limit detekcije od 1,3 μg/ml. Analizom nije dokazana prisutnost STCa niti u jednom ispitanom uzorku. Međutim u 21/58 uzoraka utvrđeno je prisustvo analita koji po
boji fluorescencije i položaju zadržavanja na TLC ploči upućuju na moguće prisustvo metabolita
STC-a. Analiza istih ekstrakata osjetljivijim kromatografskim tehnikama omogućit će precizniju
interpretaciju ovih opažanja te omogućiti detekciju nižih koncentracija STC-a u uzorcima.

Abstract (english)

Sterigmatocystin (STC) is a cytotoxic and genotoxic mycotoxin predominantly produced by molds
of the genus Aspergillus, section Versicolores. These molds are widespread in nature, but also in
indoor spaces, including those that store food and raw materials for its production, which indicates
the possibility of contamination of food and raw materials with this mycotoxin. STC contamination
has so far been identified in cereals, coffee beans, spices, nuts, animal feed, and cheese, while there
isn't much literature data on the occurrence of this mycotoxin in beer. Given the high consumption
of this popular drink in Croatia and the world, we can expect a negative effect on the health of
exposed people, due to exposure to this mycotoxin. To detect the occurrence of STC in beer, 58
samples of beer were collected from the Croatian market. Extraction was performed by combining
acetonitrile extraction with salting, and purification using SPE columns. Thus prepared extracts were
analyzed by thin-layer chromatography with a limit of detection of 1,3 μg/mL. The STC was not
detected in any of the tested samples. Taking into account resemblence in fluorescence and the spot
on chromatograms 21/58 samples may be related to the metabolates of STC. Further analysis of the
same extracts by application of more sensitive chromatographic techniques will allow a more
accurate interpretation of these observations and allow the detection of lower STC concentrations in
the samples.

• Keywords: sterigmatocistin; pivo; TLC; STC
• Keywords (english): sterigmatocystin; beer; TLC; STE
• Language: croatian
• URN:NBN: urn:nbn:hr:163:291806
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-06-21 15:51:54