Analiza podataka koncentracija serumskog kalprotektina i CRP-a kod COVID-19 bolesnika

master's thesis

• Title (english): Analysis of serum calprotectin and CRP concentration data in patients with COVID-19
• Author: Lucija Butorac
• Mentor: Anita Somborac Bačura (mentor)
• Mentor: Lovorka Đerek (mentor)
• Committee member: Anita Somborac Bačura (predsjednik povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Committee member: Lovorka Đerek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2022-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

COVID-19 je bolest uzrokovana virusom SARS-CoV-2. U organizam čovjeka virus ulazi preko dišnog sustava. Pneumociti opterećeni virusima oslobađaju različite citokine i upalne biljege, a posljedica je ozljeda pluća te difuzno oštećenje alveola koje na kraju može kulminirati sindromom akutnog respiratornog distresa. Kalprotektin je protein kojeg otpuštaju stimulirani neutrofili i monociti. Prilikom virusne ili bakterijske infekcije dišnog sustava povećava se ekspresija kalprotektina. CRP je protein akutne faze upale. Stimulacijom proupalnih citokina raste koncentracija CRP-a u plazmi.
Cilj ovog rada je bio analizirati rezultate serumskog kalprotektina i CRP-a dobivene imunoturbidimetrijskim metodama kako bi se uočile razlike ovisno o težini kliničke slike kod COVID-19 bolesnika, odnosno odsustvu bolesti COVID-19.
Korišteni su ostatni uzorci 143 ispitanika. Ispitanici su podijeljeni u četiri skupine ovisno o težini kliničkog stanja, stanju nakon preboljenja ili kao skupina zdravih dobrovoljaca. Koncentracije serumskog kalprotektina i CRP-a su određivane imunoturbidimetrijskim metodama na analitičkom sustavu Beckman Coulter AU 5800 (Beckman Coulter, Brea, SAD).
Osobe najveće životne dobi su bile u skupini bolesnika koji imaju najtežu kliničku sliku. Bolesnici s težim kliničkim slikama su imali visoke koncentracije serumskog kalprotektina i CRP-a, a ispitanici koji su preboljeli COVID-19 i ispitanici iz kontrolne skupine su imali niže koncentracije. Serumski kalprotektin je bio viši kod ispitanika koji su preboljeli COVID-19, nego u kontrolnoj skupini, iako ti ispitanici više nisu bili u akutnoj fazi upale. Koncentracije serumskog kalprotektina pokazale su značajnu korelaciju s CRP-om.
Veća distribucija težih oblika bolesti COVID-19 je kod osoba starije životne dobi. Koncentracije serumskog kalprotektina i CRP-a su visoke kod bolesnika s teškim kliničkim slikama. Serumski kalprotektin ostaje duže povišen kod ispitanika koji su preboljeli COVID-19, a više nisu u akutnoj fazi upale te time ne slijedi obrazac klasičnog biljega akutne faze kao što je CRP čija koncentracija nije povišena u stanjima bez akutne upale.

Abstract (english)

COVID-19 is a disease caused by the SARS-CoV-2 virus. The virus enters the human body through the respiratory system. Virus-loaded pneumocytes release various cytokines and inflammatory markers, resulting in lung injury and diffuse alveolar damage that may eventually culminate in acute respiratory distress syndrome. Calprotectin is a protein released by stimulated neutrophils and monocytes. During viral or bacterial infection of the respiratory system, the expression of calprotectin increases. CRP is an acute phase reactant. Stimulation of proinflammatory cytokines increases the concentration of CRP in plasma.
The aim of this study was to analyze the results of serum calprotectin and CRP determined by immunoturbidimetric methods in order to detect differences depending on the severity of the clinical picture in COVID-19 patients or the absence of COVID-19 disease.
The remaining samples of 143 subjects were used. Subjects were divided into four groups depending on the severity of the clinical condition, the condition after recovery, or as a group of healthy volunteers. Serum calprotectin and CRP concentrations were determined by immunoturbidimetric methods on the Beckman Coulter AU 5800 analytical system (Beckman Coulter, Brea, USA).
The oldest persons were in the group of patients with the most severe clinical picture. Patients with more severe clinical pictures had high concentrations of serum calprotectin and CRP, while subjects who recovered from COVID-19 and subjects in the control group had lower concentrations. Serum calprotectin was higher in subjects who recovered from COVID-19 than in the control group, although these subjects were no longer in the acute phase of inflammation. Serum calprotectin concentrations showed a significant correlation with CRP.
There is a greater distribution of more severe forms of COVID-19 disease in the elderly. Serum calprotectin and CRP concentrations are high in patients with severe clinical pictures. Serum calprotectin remains elevated for a longer period in subjects who recovered from COVID-19 and are no longer in the acute phase of inflammation, thus not following the pattern of a classic acute phase marker such as CRP whose concentration is not elevated in conditions without acute inflammation.

• Keywords: COVID-19; C-reaktivni protein; serumski kalprotektin; upala
• Keywords (english): COVID-19; C-reactive protein; serum calprotectin; inflammation
• Language: croatian
• URN:NBN: urn:nbn:hr:163:901100
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-07-19 12:25:17