Antikancerogena aktivnost nanočestica selena funkcionaliziranih ekstraktom komine masline

master's thesis

• Title (english): Anticancergenic activity of selenium nanoparticles functionalized by olive extract
• Author: Ena Vrček
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Cilj ovog rada bio je sintetizirati nanočestice selena (SeNP) procesom zelene sinteze uz dodatak
ekstrakta komine masline (EKM) koji bi potencijalno sa selenom ostvario sinergistički
antikancerogeni učinak. SeNP imaju sposobnost inhibicije rasta stanica raka, dok polifenoli EKM-a
pokazuju širok spektar povoljnih bioloških učinaka uključujući i antikancerogeni učinak. Prinosi
predloženih postupaka sinteze određeni su primjenom UV-Vis spektrofotometrije, a raspodjela
veličine čestica i zeta potencijal metodama dinamičkog i elektroforetskog raspršenja svjetlosti.
Antikancerogeni učinak je ispitan na staničnim linijama ljudskog karcinoma debelog crijeva (Caco2) i jetrenih tumorskih stanica (HepG2) pomoću MTT testa. Dobivena je unimodalna raspodjela
veličine čestica, a prosječni promjeri dobivenih nanočestica kretali su se od 56,1 nm (za SeNP
stabilizirane PS-om i funkcionalizirane s EKM-om (fPSSeNP)) do 181,7 nm (za SeNP stabilizirane
PVP-om (PVPSeNP)) što te sustave čini osobito prikladnima za peroralnu primjenu. PVPSeNP
uzorci imaju malu apsolutnu vrijednost zeta potencijala (-5,9 i -10,3 mV), dok su PSSeNP uzorci
bili karakterizirani puno negativnijim nabojem (-33 i -30 mV) što može ukazivati na veću stabilnost
PSSeNP uzoraka. Najveću toksičnost za HepG2 staničnu liniju pokazao je natrijev selenit, dok su
za Caco-2 staničnu liniju PSSeNP uzorci bili najtoksičniji. Funkcionalizacija SeNP EKM-om
reducirala je, odnosno pojačala toksičnost SeNP na HepG2, odnosno Caco-2 staničnoj liniji, što
ukazuje na različite mehanizme toksičnosti SeNP na različitim staničnim linijama.

Abstract (english)

The aim of this study was to synthesize selenium nanoparticles (SeNP) by the process of green
synthesis with the addition of olive pomace extract (OPE), which would potentially achieve a
synergistic anticancer effect with selenium. SeNPs have the ability to inhibit the growth of cancer
cells, while OPE polyphenols show wide spectrum of biological effects, includin anti-cancer
efficiency. Yields of proposed green synthesis processes were determined using UV-Vis
spectrophotometry, and the particle size distribution and zeta potential were determined using the
dynamic- and electrophoretic light scattering methods. The anticancer efficiency was tested on
human colon cancer cell lines (Caco-2) and liver tumor cells (HepG2) using the MTT test. A
unimodal particle size distribution was obtained, and the average diameters of the obtained
nanoparticles ranged from 56.1 nm (for SeNP stabilized with PS and functionalized with OPE
(fPSSeNP)) to 181.7 nm (for SeNP stabilized with PVP (PVPSeNP)) which makes these systems
particularly suitable for oral administration. PVPSeNP samples have a small absolute value of zeta
potential (-5.9 and -10.3 mV), while PSSeNP samples were much more negatively charged (-33 and
-30 mV) which may indicate a higher stability of PSSeNP samples. Sodium selenite showed the
highest toxicity for the HepG2 cell line, while for the Caco-2 cell line PSSeNP samples were the
most toxic. Functionalization of SeNP with OPE reduced and increased the toxicity of SeNP on
HepG2 and Caco-2 cell lines, respectively, which also indicates different mechanisms of SeNP
toxicity in different cell lines.

• Keywords: nanočestice; sinteza; polivinilpirolidon; polisorbat 20; biološki otpad; Caco-2 stanice; HepG2 stanice
• Keywords (english): nanoparticles; synthesis; polyvinylpyrrolidone; polysorbate 20; biological waste; Caco-2 cells; HepG2 cells
• Language: croatian
• URN:NBN: urn:nbn:hr:163:856581
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-07-14
• Created on: 2022-07-20 08:12:49