Optimizacija izrade i biološki učinci kumestrolom bogatih glicerolnih ekstrakata zeleni hmeljaste vije (Medicago lupulina L.)

master's thesis

• Title (english): Optimization of preparation and biological activity of coumestrol-rich glycerol exctracts of black medick (Medicago lupulina, L.)
• Author: Valentina Poljak
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Sanda Vladimir-Knežević (član povjerenstva)
• Committee member: Renata Jurišić Grubešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2020-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Biljni ekstrakti intenzivno se istraţuju zbog svojih mnogobrojnih učinaka na koţu te njihove potencijalne primjene u izradi dermatoloških pripravaka. U ovom diplomskom radu cilj je bio razviti metodu za ultrazvučnu ekstrakciju kumestrola iz zeleni hmeljaste vije koristeći glicerol, netoksično i biorazgradivo otapalo dostupno iz obnovljivih izvora. TakoĎer, imajući u vidu potencijalnu primjenu kumestrolom bogatih ekstrakata zeleni hmeljaste vije u dermatološkim pripravcima, ispitani su njihovi biološki učinci. Ekstrakcijski pokusi planirani su pomoću Box-Behnkenovog dizajna sa sljedećim rasponom ispitivanih parametara: maseni udio glicerola: 10-90%, temperatura: 20-80 °C i trajanje ekstrakcije: 20-60 minuta. Sadrţaj kumestrola u ekstraktima odreĎen je RP-HPLC-DAD metodom. Višestrukom regresijskom analizom i analizom varijance (ANOVA) utvrĎeno je da se odnos izmeĎu varijabli najbolje moţe izraziti kvadratnom jednadţbom, a njeno rješenje daje optimalne uvjete za ekstrakciju kumestrola: temperatura od 80 °C, maseni udio glicerola od 90 % i trajanje ekstrakcije od 60 minuta. Statistički najznačajnije varijable za ekstrakciju bile su temperatura i maseni udio glicerola čije je povećanje rezultiralo povećanom koncentracijom kumestrola. Nadalje, biološki učinci inhibicije lipoksigenaze, tirozinaze, i elastaze ispitani su enzimskim testovima, a antioksidativna aktivnost β-karoten linoleatnom analizom. Ekstrakt je pokazao umjerenu sposobnost inhibicije lipoksigenaze te blagu inhibiciju tirozinaze i kolagenaze. Pokazan je iznimno dobar antioksidativni potencijal ekstrakta.

Abstract (english)

Herbal extracts are intensively researched due to their numerous effects on the skin and their potential application in the production of dermatological preparations. The aim of this thesis was to develop a method for ultrasonic extraction of cumestrol from black medick using glycerol, a non-toxic and biodegradable solvent available from renewable sources. Having in mind the potential application of cumestrol-rich extracts of black medick in dermatological preparations, their biological effects were investigated. Extraction experiments were planned using the Box-Behnken design with the following range of test parameters: glycerol content: 10-90% (w/w), temperature: 20-80 °C and extraction duration: 20-60 minutes. The content of cumestrol in the extracts was determined by RP-HPLC-DAD method. Multiple regression analysis and analysis of variance (ANOVA) found that the relationship between the variables can best be expressed by a quadratic equation, and its solution provides optimal conditions for cumestrol extraction: temperature of 80 °C, 90% glycerol and extraction duration of 60 minutes. Statistically, the most significant variables for extraction were temperature and glycerol content whose increase resulted in increased cumestrol concentration. Furthermore, the biological effects of lipoxygenase, tyrosinase, and elastase inhibition were examined by enzymatic assays, and the antioxidant activity by β-carotene linoleate analysis. The extract showed moderate ability to inhibit lipoxygenase and mild inhibition of tyrosinase and collagenase, with notable antioxidant potential.

• Keywords: hmeljasta vija; kumestrol; ultrazvučna ekstrakcija; glicerol; Box-Behnken dizajn; lipoksigenaza; tirozinaza; elastaza; β-karoten linoleatna analiza; antioksidativna aktivnost
• Keywords (english): black medick; coumestrol; ultrasound-assisted extraction; glycerol; Box-Behnken design; lipoxygenase; tyrosinase; elastase; β-carotene linoleate analysis; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:359647
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-08-26 13:46:26