Interakcije prehrambenih vlakana s polifenolima iz biljnih ekstrakata: utjecaj na biodostupnost

master's thesis

• Title (english): Interactions of dietary fiber with polyphenols from plant extracts: influence on bioavailability
• Author: Marina Eršek
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Nova saznanja o ulozi hrane u prevenciji i liječenju bolesti dovela su do razvoja nove/funkcionalne hrane koja, osim esencijalnih nutrijenata nužnih za zadovoljavanje fizioloških potreba organizma, sadrži i sastavnice za koje je dokazano da imaju važnu ulogu u prevenciji i liječenju kroničnih oboljenja. Prehrambena vlakna i biljni ekstrakti koji obiluju antioksidansima, prvenstveno polifenolima, često se kao funkcionalne sastavnice dodaju u konačni proizvod. Međutim, u uvjetima probavnog trakta polifenoli se mogu vezati za frakciju prehrambenih vlakana što na kraju može rezultirati smanjenom oralnom biodostupnosti. Navedene interakcije su vrlo specifične i njihov intenzitet značajno varira u ovisnosti o fizikalno – kemijskim svojstvima vlakana i fenolnog spoja. U ovom radu istražene su interakcije različitih mješavina probavnih vlakana i polifenola iz ekstrakata sjemenki grožđa te ekstrakata biljke Polygonum cuspidatum u in vitro simuliranim uvjetima probavnog trakta. Biodostupnost polifenola nakon provedene digestije mjerena je Folin-Ciocalteu metodom. Ekstrakti biljke Polygonum cuspidatum, koji obiluju resveratrolom, pogodniji su za kombiniranje s prehrambenim vlaknima od ekstrakata sjemenki grožđa. Značajna razlika u afinitetu vezanja za vlakna utvrđena je za ekstrakte sjemenki grožđa od dva različita proizvođača što ukazuje na njihov različiti kemijski sastav.

Abstract (english)

New knowledge about the role of food in the prevention and treatment of diseases has led to the development of new/functional food, which, in addition to the essential nutrients necessary to meet the physiological needs of the body, also contains components that have been proven to play an important role in the prevention and treatment of chronic illnesses. Dietary fiber and plant extracts that are rich in antioxidants, primarily polyphenols, are often added as functional components to the final product. However, under the conditions of the digestive tract, polyphenols can bind to the dietary fiber fraction, which can ultimately result in reduced oral bioavailability. Mentioned interactions are very specific and their intensity varies significantly depending on the physico-chemical properties of the fiber and the phenolic compound. In this paper, the interactions of different mixtures of digestive fiber and polyphenols from grape seed extracts and Polygonum cuspidatum plant extracts were investigated in in vitro simulated conditions of the digestive tract. Bioavailability of polyphenols after digestion was measured by the Folin-Ciocalteu method. Polygonum cuspidatum plant extracts, which are rich in resveratrol, are more suitable for combining with dietary fiber than grape seed extracts. A significant difference in fiber binding affinity was found for grape seed extracts from two different manufacturers, indicating their different chemical composition.

• Keywords: prehrambena vlakna; polifenoli; biljni ekstrakti; sjemenke grožđa; Polygonum cuspidatum; resveratrol; in vitro gastrointestinalna digestija; biodostupnost
• Keywords (english): dietary fibers; polyphenols; plant extracts; grape seed; Polygonum cuspidatum; resveratrol; in vitro gastrointestinal digestion; bioavailability
• Language: croatian
• URN:NBN: urn:nbn:hr:163:473445
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-09-09 14:13:44