Procjena zajedničkog djelovanja nanočestica srebra i polistirena na modelne stanične linije imunosnog sustava

doctoral thesis

• Title (english): Evaluation of combined effects of silver and polystyrene nanoparticles on model immune cells
• Author: Krunoslav Ilić
• Mentor: Petra Turčić (mentor)
• Mentor: Ivan Pavičić (komentor)
• Committee member: Dragomira Majhen (predsjednik povjerenstva)
• Committee member: Tin Weitner (član povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacology); Zagreb
• Defense date and country: 2022-10-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy
• Universal decimal classification (UDC): 615 - Pharmacology. Therapeutics. Toxicology

Abstract

Nanotehnologija danas ima ulogu rastuće važnosti u razvoju novih industrijskih i medicinskih proizvoda. Nanočestice srebra (nanoAg) jedne su od najviše istraživanih tipova nanomaterijala za potencijalnu primjenu u medicini zbog svojih izraženih antimikrobnih svojstava. Njihova uporaba u medicinskom kontekstu ograničena je zbog štetnog utjecaja kojeg mogu imati na ljudske stanice i tkiva. Iako je toksičnost nanočestica srebra dobro poznata i istražena, manjkaju informacije o kombiniranim biološkim učincima nanoAg i drugih materijala ili nanomaterijala. Za razvoj novih, sigurnih uređaja i pomagala u kojima se koristi nanoAg, nužno je ispitati zdravstvene rizike koji mogu nastati međudjelovanjem nanoAg i materijala koji se primjenjuju u tim proizvodima. Polistiren je biološki inertan materijal široke industrijske i medicinske primjene, te su zbog toga nanočestice polistirena (nanoPS) odabrane za ispitivanje učinka mješavina nanočestica na modelne stanice ljudskog imunosnog sustava, Jurkat i THP-1. Nanočestice su karakterizirane metodama atomske mikroskopije sila te dinamičkog i elektroforetskog raspršenja svjetlosti. Analiziran je utjecaj nanoAg i nanoPS na preživljenje stanica, pokretanje apoptoze, parametre oksidativnog stresa, oštećenje DNA, pokretanje upalnog odgovora te promjene mehaničkih svojstava stanica. Unos nanočestica vizualiziran je konfokalnim mikroskopom i kvantificiran protočnim citometrom. Pokazano je da nanoAg i nanoPS ne stupaju u interakcije u vodenoj suspenziji ili u staničnom mediju, te da su mješavine nanoAg i nanoPS imale štetniji učinak na stanice Jurkat i THP-1 nego pojedinačne vrste nanomaterijala. Ovi rezultati važan su doprinos razumijevanju učinaka nanomaterijala na ljudsko zdravlje i razvoju novih i sigurnih primjena nanotehnologije u medicini.

Abstract (english)

Nanotechnology has a role of growing importance in development of novel industrial and medical applications. Silver nanoparticles (nanoAg) are some of the most researched types of nanomaterials for potential use in medicine due to their significant antimicrobial properties. Their use in medical applications is limited due to their potential toxicity to human cells and tissue. Although the toxicity of silver nanoparticles is well known, there is a distinct lack of information on combined biological effects of nanoAg and other types of materials or nanomaterials. In order to develop safe nanoAg-enabled products, it is paramount to determine if interactions of nanoAg with other materials could pose additional health hazard compared to nanoAg alone. Polystyrene is a biologically inert material with widespread use in industrial and medical applications, and polystyrene nanoparticles (nanoPS) were used to assess the effects of nanometrial mixtures on model immune cell lines, Jurkat and THP-1. Nanoparticles were characterized using atomic force microscopy, as well as dynamic and electrophoretic light scattering techniques. The effects of nanoAg and nanoPS on cell survival, induction of apoptosis, oxidative stress parameters, DNA damage, immune response and changes in cell mechanical properties were analyzed. Nanoparticle uptake was visualized with confocal microscope and quantified using flow cytometry. Results have shown that nanoAg and nanoPS do not interact in water suspensions or in cell culture media, and that nanoAg/nanoPS mixtures had an augmented, detrimental impact on Jurkat and THP-1 cells compared to individual nanoparticle types. These results present an important contribution for understanding impact of nanomaterials on human health, and for development of novel and safe applications of nanotechnology in medicine.

• Keywords: nanočestice srebra; nanočestice polistirena; stanična biologija; upalni odgovor; oksidativni stres; nanomehanika
• Keywords (english): silver nanoparticles; polystyrene nanoparticles; cell biology; immune response; oxidative stress; nanomechanics
• Language: croatian
• URN:NBN: urn:nbn:hr:163:466335
• Promotion: 2022
• Study programme: Title: Pharmacy and biochemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor znanosti (doktor znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-08 16:55:13