Gastrointestinalna stabilnost polifenola iz kompleksnih biljnih ekstrakata

master's thesis

• Title (english): Gastrointestinal stability of polyphenols from complex plant extracts
• Author: Karla Gračanin
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Kristina Radić (član povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2022-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U kreiranju funkcionalne hrane i dodataka prehrani, sve se češće koriste kompleksni biljni ekstrakti bogati polifenolima koji pokazuju širok spektar poželjnih bioloških učinaka, ponajprije antioksidativnog.
U ovom radu ispitana je antioksidativna učinkovitost i gastrointestinalna stabilnost/biodostupnost polifenola iz različitih formulacija ekstrakata sjemenki grožđa i ekstrakata japanskog dvornika. Cilj istraživanja bio je odabrati ekstrakte koji su pogodni za daljnju primjenu u kreiranju funkcionalne hrane obogaćene polifenolima. Kako bi se odredio potencijal analiziranih ekstrakata kao nutraceutika potrebno je uzeti u obzir njihovu antioksidativnu aktivnost i gastrointestinalnu stabilnost.
Antioksidativna aktivnost analiziranih uzoraka istražena je Folin-Ciocalteu (ukupni redukcijski potencijal) i TEAC metodom (antiradikalna učinkovitost). Gastrointestinalna stabilnost (biodostupnost) polifenola u analiziranim ekstraktima određena je in vitro statičkom simulacijom probave prema optimiranom INFOGEST protokolu.
Različite formulacije ekstrakata sjemenki grožđa bile su međusobno usporedive po vrijednostima antioksidativnog potencijala dok su se analizirani ekstrakti japanskog dvornika značajno razlikovali. Antioksidativni potencijal ekstrakta japanskog dvornika značajno je niži od antiradikalnog potencijala ekstrakta sjemenki grožđa. Polifenolni biljni ekstrakti bili su uglavnom stabilni u simuliranim uvjetima probavnog trakta te je malo smanjenje biodostupnosti polifenola primijećeno samo u jednoj formulaciji ekstrakta sjemenki grožđa.

Abstract (english)

Complex plant extracts rich in polyphenols are being increasingly used as bioactive ingredients in the formulation of functional food and nutritional supplements, due to the wide spectrum of their biological effects, primarily, antioxidant. .
In this paper, antioxidant activity and gastrointestinal stability of polyphenols from different formulations of grape seed extract and Polygonum Cuspidatum extract were investigated. The goal was to establish which extracts were suitable for the further use in designing polyphenol-enriched functional foods. To determine the potential of analysed extracts as nutraceuticals, both, their antioxidant activity and gastrointestinal stability need to be taken into account.
Antioxidant activity of analysed samples was investigated by Folin-Ciocalteu (total reduction potential) and TEAC method (antiradical efficiency). Gastrointestinal stability/bioaccessibility of polyphenols in analysed extracts was determined by in vitro static simulation of digestion according to the optimized INFOGEST protocol
Different formulations of grape seed extracts were comparable to each other in terms of antioxidant potential, while the analysed Polygonum cuspidatum extracts differed significantly. The antioxidant potential of the Polygonum cuspidatum extract was significantly lower in comparison to antiradical potential of the grape seed extract. It was determined that polyphenolic plant extracts were stable under simulated conditions of the digestive tract; the small decrease of the polyphenolic bioaccessibility was observed only in one analysed formulation of grape seed extract.

• Keywords: polifenoli; biodostupnost; gastrointestinalna stabilnost; kompleksni biljni ekstrakti; ekstrakt sjemenki grožđa; ekstrakt biljke Polygonum cuspidatum; resveratrol
• Keywords (english): polyohenols; bioavailability; gastrointestinal stability; complex plant extracts; grape seed extract; Polygonum cuspidatum extract; resveratrol
• Language: croatian
• URN:NBN: urn:nbn:hr:163:045482
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-29 15:21:19