Ispitivanje toksičnosti na staničnim kulturama

Professional paper - Review paper

• Author: Stipe Mustać
• Author: Domagoja Kifer
• Author: Ana-Marija Domijan
• Author: Ana Marija Marjanović Čermak
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biophysics)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

In recent years significant efforts have been made to reduce the
number of studies on experimental animals used for toxicity
testing of new compounds. In addition to the already existing 3R principle (replacement, reduction, refinement), the aim was to develop alternative in vitro
methods that would enable the same level of protection as in vivo research after
successful validation process. T herefore, special emphasis was placed on cell cultures
used in toxicology studies. This review paper gives insight into the cell
cultures used in such assays, the most commonly used cytotoxicity assays as well
as statistical models that can be used to determine the toxicity of a test compound.
In addition, guidelines of the regulatory bodies according to which such
research should be conducted are also presented in this paper.

• Keywords (croatian): stanične kulture; in vitro modeli; toksičnost
• Language: croatian
• Publication type: Professional paper - Review paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 78, no. 1-2, pp. 1-14
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:680912
• Publication: 2022
• Type of resource: Text
• Access conditions: Open access
• Created on: 2023-03-14 13:59:45