Određivanje udjela bakra, cinka i selena u grahoricama metodom induktivno spregnute plazme s masenom spektrometrijom

master's thesis

• Title (english): Determination of copper, zinc and selenium in legumes by inductively coupled plasma mass spectrometry method
• Author: Lucija Drinovac
• Mentor: Donatella Verbanac (mentor)
• Committee member: Donatella Verbanac (predsjednik povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Committee member: Roberta Petlevski (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2023-04-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Grahorice su izrazito nutritivno vrijedna skupina namirnica. Osim ugljikohidrata, među kojima se
ističu prehrambena vlakna koja smanjuju rizik nastanka bolesti probavnog trakta, posebno
kolorektalnog karcinoma te kardiovaskularnih bolesti kao i dijabetesa, u svom sastavu imaju i
visok udio proteina koji predstavljaju izvrstan izvor esencijalne, bazične aminokiseline lizina. Uz
makronutrijente, grahorice su bogate polifenolima (izoflavonima) i saponinima. Izoflavoni su vrsta
fitoestrogena, a saponini triterpenski glikozidi koji djeluju protuupalno, antikarcinogeno,
antibakterijski te imaju protektivan učinak na jetru i kardiovaskularni sustav. U sastavu
mikronutrijenata ističu se vitamini B skupine, vitamin C i E te elementi u tragovima – cink, bakar i
selen. Njihov udio u uzorcima grahorica soje, graha, slanutka i leće određen je metodom
induktivno spregnute plazme s masenom spektrometrijom (ICP-MS). Cink, bakar i selen su
kofaktori brojnih enzima, djeluju kao antioksidansi, dio su imunosnog odgovora te je njihov
odgovarajući unos nužan za održavanje homeostaze organizma. Svako odstupanje (smanjenje ili
uvećanje) od preporučenog unosa ovih esencijalnih mikroelemenata može u konačnici rezultirati
metaboličkom neravnotežom i razvojem različitih bolesti i stanja.

Abstract (english)

Legumes are an exceptionally nutritionally valuable group of food. In addition to carbohydrates,
among which dietary fibers stand out and reduce the risk of colorectal cancer, cardiovascular
diseases, and diabetes, they also contain a high proportion of proteins, an excellent essential amino
acid lysine source. In addition to macronutrients, legumes are rich in polyphenols (isoflavones) and
saponins. Isoflavones are a type of phytoestrogen, and saponins are triterpene glycosides, which have
an important anti-inflammatory, anticarcinogenic, and antibacterial role and have a protective effect
on the liver and cardiovascular system. Some of the micronutrients that stood out were B complex
vitamins, vitamins C and E, as well as trace elements - zinc, copper and selenium. Their share in the
samples of soybeans, beans, chickpeas, and lentils was determined by Inductively Coupled Plasma
Mass Spectrometry (ICP-MS) method. Zinc, copper, and selenium are cofactors of numerous
enzymes, and together with acting as antioxidants, they have an essential part in the immune system
response. Adequate intake of the aforementioned micronutrients is necessary to maintain the
homeostasis of the organism. However, excessive, as well as insufficient intake of these
micronutrients leads to the development of various diseases.

• Keywords: bakar; cink; selen; ICP-MS; grahorice; soja; grah; slanutak; leća
• Keywords (english): copper; zinc; selenium; ICP-MS; legumes; soybean; bean; chickpea; lentil
• Language: croatian
• URN:NBN: urn:nbn:hr:163:128845
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-04-13 11:08:45