Biološka aktivnost glicerolnih ekstrakata širokolisnog trputca (Plantago major L.) bogatih polifenolima i verbaskozidom za primjenu u kozmetici

master's thesis

• Title (english): Biological activity of broadleaf plantain (Plantago major L.) glycerol extracts rich in polyphenols and verbascoside for application in cosmetics
• Author: Anđela Sladić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Biljne vrste bogate biološki aktivnim sastavnicama predmetom su brojnih istraživanja zbog svojih pozitivnih učinaka na kožu i potencijalne primjene u kozmetičkim formulacijama. U prethodnim istraživanjima razvijene su (optimizirane) dvije metode ekstrakcije nadzemnih dijelova širokolisnog trputca (P. major L., Plantaginaceae), pomoću vodene otopine glicerola i mliječne kiseline. Prvom metodom je postignuta maksimalna količina ukupnih polifenola, a drugom maksimalna količina verbaskozida u priređenim ekstraktima OPT-TP odnosno OPT-VER. U ovom radu provedeno je istraživanje antioksidativne aktivnosti priređenih ekstrakata te inhibitorne aktivnosti prema enzimima koji utječu na izgled i zdravlje kože. Antioksidativna aktivnost određena je pomoću antiradikalne 2,2-difenil-1-pikrilhidrazil (DPPH) metode, a biološki učinci ispitani su enzimskim testovima inhibicije elastaze, kolagenaze, hijaluronidaze i tirozinaze. Ispitani ekstrakti pokazali su iznimno dobar antioksidativni potencijal, nadmašivši pritom aktivnost butilhidroksianisola, koji je korišten kao pozitivna kontrola. Najbolja biološka aktivnost ekstrakata ostvarena je u testu inhibicije kolagenaze. Nadalje, utvrđena je značajna inhibicija hijaluronidaze, umjerena sposobnost inhibicije elastaze te blaga inhibicija tirozinaze. Ekstrakt OPT-TP postigao je veću aktivnost u svim biološkim ispitivanjima, osim u testu inhibicije kolagenaze, gdje je aktivniji bio OPT-VER. Također, u odnosu na OPT-TP, OPT-VER pokazao je nešto veću sposobnost hvatanja slobodnih radikala u DPPH antioksidativnoj analizi.

Abstract (english)

Plant species rich in biologically active components are the subject of numerous studies due to their positive effects on the skin and potential application in cosmetic formulations. In previous research, two methods using aqueous solution of glycerol and lactic acid were developed (optimized) for extraction of broadleaf plantain (P. major L., Plantaginaceae) aerial parts. The first method achieved maximum quantity of total polyphenols, and the other maximum quantity of verbascoside in the corresponding extracts (OPT-TP and OPT-VER). In this work, the antioxidant activity of the prepared extracts and the inhibitory activity against enzymes that affect the appearance and health of the skin were investigated. Antioxidant activity was determined using the antiradical 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and biological effects were examined by enzymatic assays of elastase, collagenase, hyaluronidase and tyrosinase inhibition. Tested extracts showed extremely notable antioxidant potential, surpassing the activity of butylhydroxyanisole, which was used as a positive control. The best biological activity of the extracts was achieved in the collagenase inhibition test. Furthermore, a significant inhibition of hyaluronidase, a moderate ability to inhibit elastase and a slight inhibition of tyrosinase was established. Extract OPT-TP achieved greater activity in all biological tests, except in the collagenase inhibition test, where OPT-VER was more active. Also, compared to OPT-TP, OPT-VER showed a slightly higher ability to capture free radicals in the DPPH antioxidant assay.

• Keywords: širokolisni trputac; polifenoli; verbaskozid; oksidativni stres; starenje kože; antioksidativna aktivnost; DPPH metoda; elastaza; kolagenaza; hijaluronidaza; tirozinaza
• Keywords (english): broadleaf plantain; polyphenols; verbascoside; oxidative stress; skin aging; antioxidant activity; DPPH method; elastase; collagenase; hyaluronidase; tyrosinase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:425084
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-06-16
• Created on: 2023-11-08 13:49:11