Određivanje biološki aktivnih tvari i antioksidativne aktivnosti u dodacima prehrani za liječenje upalnih bolesti crijeva

master's thesis

• Title (english): Determining bioactive substances and antioxidant activity in dietary supplements for the treatment of inflammatory bowel diseases
• Author: Maria Levanić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Goran Poje (član povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2023-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Upalne bolesti crijeva su kronične, idiopatske, relapsirajuće bolesti koje danas predstavljaju veliki
javnozdravstveni problem i zahvaćaju sve veći broj ljudi. Podijeljene su u dvije velike podgrupe:
Crohnova bolest i ulcerozni kolitis, ovisno o dijelu gastrointestinalnog trakta kojeg zahvaćaju upalni
procesi. Budući da su zahtjevi za biljnom terapijom među pacijentima s upalnim bolestima crijeva
sve veći zbog brojnih pozitivnih učinaka biljnih vrsta, cilj ovog rada bio je procijeniti kvalitetu
dodataka prehrani za liječenje upalnih bolesti crijeva obzirom na pojedinačne polifenole, odnosno
biološki aktivne tvari u tim dodacima, te odrediti antioksidativnu aktivnost istih. Sadržaj
pojedinačnih biološki aktivnih tvari u analiziranim uzorcima određen je tekućinskom
kromatografijom visoke djelotvornosti u kombinaciji s detektorom s nizom dioda, dok je
antioksidativna aktivnost procijenjena RPA metodom. Analizirani dodaci prehrani koji sadrže
kurkumu i indijski tamjanovac moga su se smatrati dobrim izvorom biološki aktivnih sastavnica.
Antioksidativna aktivnost monopreparata kurkume bila je veća nego kod ostalih analiziranih
monopreparata smole indijskog tamjanovca, te multipreparata kurkume, sporiša, kore limuna i crnog
papra. Nadalje, postoji značajna razlika u sadržaju DMC-a i CUR-a između analiziranih
monopreparata kurkume, kao i u sadržaju AKBA-e između analiziranih monopreparata smole
indijskog tamjanovca.

Abstract (english)

Inflammatory bowel diseases are chronic, idiopathic, relapsing conditions that pose a significant public
health problem today, affecting an increasing number of people. They are divided into two major
subgroups: Crohn's disease and ulcerative colitis, depending on the part of the gastrointestinal tract
affected by inflammatory processes. As the demand for herbal therapy among patients with
inflammatory bowel diseases is growing due to the numerous positive effects of plant species, this work
aimed to assess the quality of dietary supplements for the treatment of inflammatory bowel diseases in
terms of individual polyphenols, i.e., bioactive substances in these supplements, and to determine their
antioxidant activity. The content of individual polyphenols in the analyzed samples was determined by
high-performance liquid chromatography combined with a diode array detector, while the antioxidant
activity was evaluated using the RPA method. The analyzed dietary supplements containing turmeric
and Indian frankincense can be considered as a good source of bioactive components. The antioxidant
activity of curcumin monopreparations was higher compared to other analyzed monopreparations of
Boswellia resin, as well as multipreparations of curcumin, sage, lemon peel, and black pepper.
Furthermore, there is a significant difference in content of DMC and CUR among the analyzed curcumin
monopreparations, as well as in content of AKBA among the analyzed Boswellia resin
monopreparations.

• Keywords: polifenoli; dodaci prehrani; RPA; HPLC; upalne bolesti crijeva; kurkuma; indijski tamjanovac; crni papar
• Keywords (english): polyphenols; dietary supplements; RPA; HPLC; inflammatory bowel diseases; turmeric; Indian frankincense; black pepper
• Language: croatian
• URN:NBN: urn:nbn:hr:163:465206
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-12
• Created on: 2023-11-16 14:44:03