Određivanje stabilnosti funkcijskih testova štitnjače

master's thesis

• Title (english): The stability study of thyroid function tests
• Author: Rea Takač
• Mentor: Donatella Verbanac (mentor)
• Committee member: Donatella Verbanac (predsjednik povjerenstva)
• Committee member: Karmela Barišić (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Poremećaji funkcije štitne žlijezde u sve su većem porastu. Laboratorij, uz ultrazvučnu dijagnostiku, ima ključnu ulogu
u dijagnozi i diferencijaciji poremećaja te praćenju terapije. U tu svrhu, određuju se funkcijski testovi štitnjače TSH,
tT4, tT3, fT4, fT3, anti-TPO i anti-TG. U rutinskom radu laboratorija, ponekad je potrebno odgoditi ili naknadno odrediti
pojedine testove, zbog čega je važno znati vremenske okvire i temperaturne uvjete u kojima su testovi stabilni. Ciljevi
ovog istraživanja bili su ispitati stabilnost funkcijskih testova štitnjače u serumu u epruvetama s gelom tijekom 8 h na
sobnoj temperaturi te tijekom 72 sata na 2 – 8 ºC, s prethodnim stajanjem uzoraka seruma 8 h na sobnoj temperaturi.
Ispitivanje je provedeno u Endokrinološkom laboratoriju KBC Sestre milosrdnice, a dizajn istraživanja prilagođen je
prema uputama Radne grupe za predanalitiku EFLM-a. Na automatskom analizatoru Abbott Architect i2000 određivale
su se koncentracije TSH, tT4, tT3, fT4, fT3, anti-TPO i anti-TG u definiranim vremenskim točkama (0 h, 4 h, 8 h, 24 h,
48 h i 72 h). Stabilnost pojedinog testa je određena uspoređivanjem srednjeg postotka odstupanja (PD̅̅̅̅) u definiranim
vremenskim točkama i maksimalnog dopuštenog odstupanja (MPD/%), a rezultati su prikazani grafički. Dobiveni
rezultati pokazali su kako, za niti jedan funkcijski test, srednji postotak odstupanja u definiranim vremenskim točkama
ne premašuje maksimalno dopušteno odstupanje te je time dokazano da su funkcijski testovi štitnjače stabilni u serumu
u epruvetama s gelom 8 h na sobnoj temperaturi te tijekom 72 h na temperaturi 2 – 8 ºC s prethodnim stajanjem uzoraka
seruma 8 h na sobnoj temperaturi. Sukladno tome, određivanje funkcijskih testova štitnjače iz seruma je moguće odgoditi
ili naknadno odraditi unutar 72 h.

Abstract (english)

Nowadays, the number of thyroid gland disorders is rapidly rising. Laboratory together with ultrasound diagnostics has
a key role in diagnosis, diferentiation of disorders and therapy monitoring. Thyroid function tests TSH, tT4, tT3, fT4, fT3,
anti-TPO i anti-TG are determined for this purpose. In the routine work of the laboratory, it is sometimes necessary to
postpone or subsequently determine certain tests, which is why it is important to know the time frames and temperature
conditions in which the tests are stable. The aims of this study were to study the stability of thyroid function tests in
serum in tubes with gel during 8 h at room temperature and during 72 h at 2 – 8 ºC, with previous storage of serum
samples for 8 h at room temperature. The study was conducted in the Endocrine Laboratory of KBC Sestre milosrdnice,
and the research design was adapted according to the instructions of the Working Group for Preanalytics of EFLM. The
concentrations of TSH, tT4, tT3, fT4, fT3, anti-TPO and anti-TG were determined on the Abbott Architect i2000 automatic
analyzer at defined time points (0 h, 4 h, 8 h, 24 h, 48 h and 72 h). The stability of an individual test is determined by
comparing the mean percentage of difference (PD̅̅̅̅) at defined time points and the maximum allowed difference
(MPD/%). The results of the study are presented graphically. The obtained results showed that, for any functional test,
the mean percentage of difference at the defined time points did not exceed the maximum allowed difference, which
proves that the functional tests are stable in serum in test tubes with gel for 8 h at room temperature and for 72 h at a
temperature of 2 – 8 ºC with previous storage of the serum samples for 8 h at room temperature. Therefore, the
determination of thyroid function tests in serum can be postponed or subsequently performed within 72 hours.

• Keywords: štitnjača; stabilnost; tiroksin; trijodtironin; antitijela
• Keywords (english): thyroid gland; stability; thyroxine; triiodthyronine; antibodies
• Language: croatian
• URN:NBN: urn:nbn:hr:163:561389
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-17 10:17:20