Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60

master's thesis

• Title (english): Evaluation of erythrocytes morphological characteristics using Sysmex DI-60 digital morphology analyzer
• Author: Josipa Vučetić
• Mentor: Dunja Rogić (mentor)
• Committee member: Dunja Rogić (predsjednik povjerenstva)
• Committee member: Ana Kozmar (član povjerenstva)
• Committee member: Lara Milevoj Kopčinović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2023-07-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Poremećaji eritrocita se odražavaju karakterističnim morfološkim promjenama, stoga je bitno
detektirati i kvantificirati morfološke abnormalnosti. Hematološki analizatori daju pouzdane rezultate
kompletne krvne slike u većini slučajeva, ali u prisutnosti morfoloških promjena eritrocita još uvijek ne
mogu ponuditi cjelovitu kliničku sliku. U tu svrhu izvodi se pregled razmaza periferne krvi koji se
posljednjih godina nastoji automatizirati, a razvojem umjetne inteligencije i algoritama za digitalnu
mikroskopiju to postaje moguće. Cilj ovog rada bio je ispitati pouzdanost automatizirane procjene
morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60, kao dio
verifikacije novog uređaja za digitalnu morfologiju u svrhu uvođenja automatizirane procjene
morfoloških karakteristika eritrocita u rutinski laboratorijski rad. Ispitana je korelacija šest morfoloških
karakteristika (polikromazija, hipokromija, anizocitoza, mikrocitoza, makrocitoza, poikilocitoza)
dobivenih na uređaju za digitalnu mikroskopiju, s parametrima dobivenima na hematološkom
analizatoru Sysmex XN-3100 (Rtc, MCH, RDW, MCV). Utvrđena je umjerena korelacija između
hipokromije i MCH, mikrocitoze i MCV, anizocitoze i RDW. Slaba korelacija utvrđena je između
polikromazije i broja retikulocita te između makrocitoze i MCV. Kako bi slaganje između ovih
parametara bilo bolje, potrebno je poboljšati same algoritme digitalne mikroskopije, ali i prilagoditi
kriterije za stupnjevanje pojedinih karakteristika.

Abstract (english)

Erythrocytes' disorders are reflected in characteristic morphological changes, therefore it is
essential to detect and quantify them. Hematology analyzers provide reliable results of a complete
blood count in most cases, but in the presence of erythrocytes' morphological changes they still
cannot offer a complete clinical picture. In this case, inspection of the peripheral blood smear is
performed. In recent years, there have been attempts made to automate this process, and with the
development of artificial intelligence and algorithms for digital microscopy, this became possible.
The aim of this thesis was to test the reliability of the automated assessment of erythrocytes'
morphological characteristics using Sysmex DI-60 digital morphology analyzer. The correlation of
six morphological characteristics (polychromasia, hypochromia, anisocytosis, microcytosis,
macrocytosis, poikilocytosis) obtained on a Sysmex DI-60 with parameters obtained on a
hematology analyzer Sysmex XN-3100 (Rtc, MCH, RDW, MCV) was examined. Moderate
correlation was found between hypochromia and MCH, microcytosis and MCV, anisocytosis and
RDW. Weak correlation was found between polychromasia and reticulocytes' count as well as
between macrocytosis and MCV. In order to obtain better agreement between these parameters, it
is necessary to improve the digital microscopy algorithm, but also to adjust the criteria for grading
individual characteristics.

• Keywords: digitalna mikroskopija; eritrociti; morfologija eritrocita; Sysmex DI-60
• Keywords (english): digital microscopy; erytrocytes; erythrocytes' morphology; Sysmex DI-60
• Language: croatian
• URN:NBN: urn:nbn:hr:163:207457
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-12-07 13:14:56