Primjena rendgenske fluorescencijske spektrometrije s totalnom refleksijom za ispitivanje detoksifikacijskog potencijala zeolita

master's thesis

• Title (english): Application of total reflection X-ray fluorescence spectrometry for investigating the detoxification potential of zeolites
• Author: Ani Buljević
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2023-09-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Detoksifikacijska svojstva zeolita klinoptilolita potaknula su zanimanje za njegovu primjenu u
humanoj medicini. Popularnost zeolita, kao i broj proizvoda sa zeolitom na tržištu svakodnevno
raste. U ovoj studiji analizirani su uzorci urina kod zdravih dobrovoljaca prije i nakon konzumacije
zeolita u vremenskim intervalima od 0, 4, 8, 12 i 24 sata kako bi se ispitala njegova moć vezivanja
metalnih kationa te njegova sposobnost da se ti elementi i eliminiraju iz organizma. U tu svrhu,
korištena je analitička tehnika rendgenska fluorescencijska spektrometrija s totalnom refleksijom
(TXRF) koja se pokazala kao brza i pouzdana tehnika pomoću koje se mogu analizirati ne samo
metali, već i halogeni elementi kao što je brom. Dodatno, tehnika slijedi principe „zelene kemije“
pošto ne zahtjeva potrošnju pogonskih plinova te su potrebne male količine uzoraka i reagensa.
Elementi koji su detektirani i određivani su brom, rubidij, stroncij, mangan i cink. Svi su elementi
pokazali povećane koncentracije u ispitivanim uzorcima u odnosu na kontrolne uzorke što ukazuje
na sposobnost zeolita da adsorbira metale, ali i ostale elemente te povećava njihovu eliminaciju iz
organizma.

Abstract (english)

The detoxification properties of zeolite clinoptilolite have sparked interest in its application in
human medicine. The popularity of zeolite, as well as the number of products containing zeolite in
the market, grows on a daily basis. In this study, urine samples from healthy volunteers were
analyzed before and after zeolite consumption at time intervals of 0, 4, 8, 12 and 24 hours in order
to investigate its metal cation binding capacity and its ability to eliminate these elements from the
organism. For this purpose, an analytical technique of X-ray fluorescence spectrometry with total
reflection (TXRF) was used, which proved to be a fast and reliable technique capable of analysing
not only metals but also halogen elements such as bromine. In addition, the technique follows the
principles of ‘green chemistry’ as it does not require the consumption of drive gases and only small
quantities of samples and reagents are needed. The elements detected and determined were bromine,
rubidium, strontium, manganese, and zinc. All elements showed increased concentrations in the test
samples compared to control samples, indicating the ability of zeolites to adsorb metals as well as
other elements and enhance their elimination from the organism.

• Keywords: zeolit klinoptilolit; rendgenska fluorescencijska spektrometrija s totalnom refleksijom; uzorci urina
• Keywords (english): zeolite clinoptilolite; total reflection X-ray fluorescence; urine samples
• Language: croatian
• URN:NBN: urn:nbn:hr:163:085050
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-11
• Created on: 2024-01-04 15:31:53