Ispitivanje biološke aktivnosti ekstrakata primorskog vriska (Satureja montana L.) bogatih flavonoidima za primjenu u fitofarmaciji i kozmetici

master's thesis

• Title (english): Examination of the biological activity of extracts of winter savory (Satureja montana L.) rich in flavonoids for use in phytopharmacy and cosmetics
• Author: Josip Cvetko
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-10-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Izgledu i zdravlju ljudske kože oduvijek se obraćala velika pozornost i briga. Oksidativni stres, starenje, sunčeve UV zrake i upalne bolesti epidermisa i dermisa uzokuju neželjene promjene ljudske kože koje se liječe i ispravljaju kozmetičkim pripravcima. Trenutno postojeće sastavnice kozmetičkih proizvoda imaju mogućnost ispoljavanja nuspojava pa postoji potreba za otkrivanjem novih terapijskih mogućnosti iz prirodnih izvora. Ekstrakti primorskog vriska dobiveni ekstrakcijom s glicerolom i HP-β-CD sadrže visoke koncentracije flavonoida i posebice luteolin-7-O-glukozida. U ispitivanjima biološke aktivnosti ekstrakti su ostvarili izvrsnu antioksidativnu aktivnost (ispitanu pomoću DPPH metode i redukcijske snage) i protuupalno djelovanje (određeno ispitivanjem inhibicije lipoksigenaze i denaturacije ovalbumina). Također, ispitana je inhibitorna aktivnost ekstrakata na enzimima ljudske kože tirozinazi, elastazi i hijaluronidazi. Dok za tirozinazu i elastazu ekstrakti nisu ostvarili značajan stupanj inhibicije, ekstrakti su uspješno inhibirali enzim hijaluronidazu. Zahvaljujući tome, ekstrakti vrste S. montana mogu se razmotriti kao potencijalne nove komponente u budućim kozmetičkim pripravcima namijenjenima očuvanju zdravlja i postizanju željenog izgleda kože.

Abstract (english)

Great attention and care have always been paid to the appearance and health of human skin. Oxidative stress, aging, the sun's UV rays and inflammatory diseases of the epidermis and dermis cause unwanted changes in human skin that are treated and corrected with cosmetic preparations. The currently existing components of cosmetic products have the possibility of side effects, so there is a need to discover new therapeutic possibilities from natural sources. Winter savory extracts obtained by extraction with glycerol and HP-β-CD contain high concentrations of flavonoids and luteolin-7-O-glucoside in particular. In biological activity tests, the extracts achieved excellent antioxidant activity (tested by DPPH method and reducing power) and anti-inflammatory activity (determined by lipoxygenase inhibition and ovalbumin denaturation tests). The inhibitory activity of extracts on human skin enzymes tyrosinase, elastase and hyaluronidase was also tested. While the extracts did not achieve a significant degree of inhibition of tyrosinase and elastase, they successfully inhibited the enzyme hyaluronidase. Thus, the extracts obtained from S. montana can be considered as potential new components in future cosmetic preparations intended to preserve health and achieve the desired appearance of the skin.

• Keywords: Satureja montana; primorski vrisak; hidroksipropil-β-ciklodekstrin; glicerol; antioksidativna aktivnost; protuupalna aktivnost; tirozinaza; elastaza; hijaluronidaza
• Keywords (english): Satureja montana; winter savory; hydroxypropyl-β-cyclodextrin; glycerol; antioxidant activity; anti-inflammatory activity; tyrosinase; elastase; hyaluronidase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:777015
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-10-26
• Created on: 2024-01-25 13:54:34