Određivanje bioaktivnih sastavnica u komercijalnim ekstraktima vrste Punica granatum L.

master's thesis

• Title (english): Identification and quantification of bioactive compounds in commercially available Punica granatum L. extracts
• Author: Lovro Premelč
• Mentor: Maja Bival Štefan (mentor)
• Committee member: Maja Bival Štefan (predsjednik povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Committee member: Marijana Zovko Končić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-07-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada istraživane su bioaktivne sastavnice šest komercijalnih ekstrakata
vrste Punica granatum L. Tankoslojnom kromatografijom (TLC) nije dokazana značajna prisutnost
flavonoida i fenolnih kiselina. UV-Vis spektrofotometrijskom metodom određen je sadržaj
flavonoida i fenolnih kiselina koji je iznosio redom 0,0009 – 0,0119 % i 0,1275 – 1,1041 %.
Tekućinskom kromatografijom visoke djelotvornosti (HPLC) u uzorcima koncentracije 5 mg/mL
nije detektiran vitamin C. Antiradikalna aktivnost uzoraka dokazana je sposobnošću hvatanja DPPH
i NO radikala.

Abstract (english)

Bioactive compounds of six commercially available Punica granatum L. extracts were examined in
this thesis. Thin Layer Chromatography (TLC) did not show significant amount of flavonoids and
phenolic acids. Quantification of flavonoids and phenolic acids was done using UV-Vis
spectrophotometry and the amounts ranged from 0,0009 – 0,0119 % and 0,1275 – 1,1041 %,
respectively. High-performance liquid chromatography (HPLC) was used to quantify vitamin C in
5 mg/mL extract solutions and did not detect any. Antiradical activity was proven with DPPH and
NO scavenging methods.

• Keywords: Punica granatum; flavonoidi; fenolne kiseline; TLC; UV-Vis; vitamin C; HPLC; DPPH; NO
• Keywords (english): Punica granatum; Flavonoids; Phenolic acids; TLC; UV-Vis; Vitamin C; HPLC; DPPH; NO
• Language: croatian
• URN:NBN: urn:nbn:hr:163:639673
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-19
• Created on: 2024-03-21 13:06:10