Primjena 3D staničnih kultura u istraživanju plućnih bolesti

master's thesis

• Title (english): The application of 3D cell cultures in pulmonary disease research
• Author: Antonija Kovačić
• Mentor: Anita Somborac Bačura (mentor)
• Committee member: Anita Somborac Bačura (predsjednik povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2023-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

U posljednjim desetljećima, napredak u području medicine i biotehnologije omogućio je razvoj inovativnih tehnika za istraživanje i liječenje raznih bolesti. Jedno od područja koje je doživjelo značajan napredak jest istraživanje plućnih bolesti, a trodimenzionalne (3D) stanične kulture su postale ključni alat u istraživanju ovih patoloških stanja. Primjena 3D staničnih kultura u istraživanju plućnih bolesti otvara nove perspektive za razumijevanje, dijagnostiku i liječenje ovih kompleksnih bolesti. Ovi napredni in vitro modeli omogućuju znanstvenicima bolje modeliranje pluća u laboratorijskim uvjetima, razumijevanje patogeneze i ispitivanje potencijalnih terapijskih pristupa. 3D stanične kulture uvelike su korištene u širokom rasponu, uključujući istraživanje napredovanje raka, razvoj tkiva i metabolizam lijekova. Tradicionalni dvodimenzionalni (2D) sistemi stanične kulture imaju ograničenja u oponašanju kompleksne 3D okoline plućnog tkiva. Nasuprot tome, 3D stanične kulture pružaju model koji je fiziološki relevantniji za proučavanje plućnih bolesti, a budućnost istraživanja plućnih bolesti uvelike ovisi o daljnjem razvoju 3D staničnih kultura.

Abstract (english)

In recent decades, progress in the field of medicine and biotechnology has enabled the development of innovative techniques for research and treatment of various diseases. One of the areas that has progressed significantly is the research of lung diseases, and three-dimensional (3D) cell cultures have become a key tool in the research of these pathological conditions. The application of 3D cell cultures in the research of lung diseases opens new perspectives for the understanding, diagnosis and treatment of these complex diseases. These advanced in vitro models allow scientists to model the lung in laboratory conditions, understand the pathogenesis and test potential therapeutic approaches. The 3D cell cultures have been used extensively over a wide range of biological processes, including cancer progression, tissue development, and drug metabolism. Traditional two-dimensional (2D) cell culture systems have limitations in mimicking the complex 3D environment of lung tissue. In contrast, 3D cell culture systems provide a more physiologically relevant model to study pulmonary diseases and the future of lung disease research depends largely on the further development of 3D cell cultures.

• Keywords: 3D stanične kulture; 2D stanične kulture; organoidi; sferoidi; granica medij-zrak; pluća na čipu; plućne bolesti
• Keywords (english): 3D cell culture; 2D cell culture; organoids; spheroids; air-liquid interface; lung on a chip; pulmonary diseases
• Language: croatian
• URN:NBN: urn:nbn:hr:163:754177
• Study programme: Title: Medical biochemistry - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-03-21 13:50:17