Usporedba ICP-MS i TXRF tehnike za elementarnu analizu proteina sirutke

master's thesis

• Title (english): Comparison of ICP-MS and TXRF techniques for elemental analysis of whey protein
• Author: Lucija Budić
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2024-06-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Proteini sirutke jedni su od često upotrebljavanih dodataka prehrani te stoga određivanje njihovog mineralnog sastava može biti od velikog interesa za potrošače te od koristi za proizvođače. U mineralnom je sastavu proteina sirutke dokazana prisutnost teških metala. S obzirom na moguće štetne utjecaje na ljudsko zdravlje, korisno je odrediti sadržaj prisutnih teških metala u proteinima sirutke. Glavni cilj ovog eksperimentalnog diplomskog rada bio je odrediti elementarni sastav proteina sirutke dostupnih na tržištu pomoću TXRF i ICP-MS tehnike te usporediti rezultate dobivene dvjema različitim tehnikama. Prikupljeni uzorci pripremljeni su postupkom mikrovalne digestije. U svim uzorcima primjenom obje tehnike određeni elementi su: željezo, bakar, cink, stroncij, rubidij. U svim uzorcima primjenom TXRF tehnike određen je i brom. Drugim spektroskopskim tehnikama brom nije jednostavno odrediti što čini još jednu prednost ove tehnike u usporedbi s ostalim spektroskopskim tehnikama za elementarnu analizu. Analizom rezultata uočene su značajne razlike u sadržaju elemenata u različitim uzorcima proteina sirutke. U svim uzorcima najzastupljeniji element je željezo, a nakon njega većinom slijedi cink zatim bakar ili stroncij. Dobiveni rezultati s obje tehnike u većini slučajeva nisu pokazali statističku značajnu razliku, što potvrđuje da se umjesto standardizirane ICP-MS tehnike kao alternativa u određivanju sadržaja proteina sirutke može koristiti i TXRF. Primjena TXRF tehnike prvenstveno zbog uporabe vrlo male količine uzorka i reagensa, nekorištenja rashladnih sustava i plinova, kvantifikacije pomoću unutarnjeg standarda je jednostavnija, brža te ekonomski i ekološki održivija tehnika u usporedbi s ICP-MS tehnikom. Obećavajući rezultati dobiveni primjenom ove tehnike za određivanje sadržaja teških metala u proteinima sirutke otkrivaju veliki potencijal ovog uređaja za brzu i jednostavnu analizu različitih tipova uzoraka.

Abstract (english)

Whey proteins are among the most commonly used dietary supplements, so determining their mineral composition can be of great interest to consumers and benefit to manufacturers. The presence of heavy metals has been detected in the mineral composition of whey proteins. Considering the possible harmful effects on human health, it is useful to determine the concentration of heavy metals present. The main objective of this experimental work was to determine the elemental composition of whey proteins available on the market using the TXRF and ICP-MS techniques and to compare the results obtained with the two different techniques. The following elements were determined in all samples using both techniques: Iron, Copper, Zinc, Strontium, Rubidium. Bromine was also determined in all samples using the TXRF technique. Bromine is difficult to determine using other spectroscopic techniques, which represents a further advantage of this technique over other spectroscopic techniques for elemental analysis. The analysis of the results showed considerable differences in the element content of the different whey protein samples. Iron was the most abundant element in all samples, followed by zinc, copper and strontium. The results obtained with both techniques showed no statistically significant difference in most cases, confirming that the TXRF technique can be used as an alternative to the standardised ICP-MS technique for the determination of whey protein content Compared to the ICP-MS technique, the TXRF technique is simpler, faster, more economical and more environmentally friendly, as only a very small amount of samples and reagents are required, no cooling systems or gases are used and quantification is performed with an internal standard. The promising results obtained with this technique in the determination of heavy metal content in whey proteins show the great potential of this device for fast and easy analysis of different types of samples.

• Keywords: protein sirutke; multielementna analiza; TXRF; ICP-MS
• Keywords (english): whey protein; multielement analysis; TXRF; ICP-MS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:111600
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-06-20
• Created on: 2024-07-26 08:55:20