Primjenjivost modificirane QuEChERS metode za detekciju mikotoksina sterigmatocistina u krvnoj plazmi zdravih darivatelja krvi

master's thesis

• Title (english): Applicability of the modified QuEChERS method for the detection of the mycotoxin sterigmatocystin in the blood plasma of healthy blood donors
• Author: Ana Perišić
• Mentor: Daniela Jakšić (mentor)
• Committee member: Daniela Jakšić (predsjednik povjerenstva)
• Committee member: Maja Šegvić Klarić (član povjerenstva)
• Committee member: Miranda Sertić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2024-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Podaci o izravnoj izloženosti ljudi mikotoksinu sterigmatocistinu (STC) su ograničeni, iako je STC vrlo prisutan u zatvorenim prostorima. Stoga smo odlučili provesti analizu krvne plazme dobrovoljnih darivatelja krvi kako bismo procijenili izloženost zdravih osoba ovom mikotoksinu. Metoda za ekstrakciju STC-a iz uzoraka krvne plazme (N = 94) slijedila je modificiranu QuEChERS metodu, u kojoj je ekstrakcija zakiseljenim acetonitrilom kombinirana s dodatkom soli i pročišćavanjem putem SPE kolona. Pripremljeni ekstrakti analizirani su metodom LC-MS/MS. Analiza je potvrdila prisutnost STC-a u 21/94 uzoraka, ali samo u tragovima, iz čega se može zaključiti da izloženost STC-u ne predstavlja zdravstveni rizik u populaciji obuhvaćenoj istraživanjem. U budućnosti bi se ova metoda mogla koristiti za procjenu izloženosti ljudi i životinja STC-u i evaluaciju njegove toksikokinetike. To bi bilo osobito važno za osobe koje borave u područjima gdje su otkrivene povećane koncentracije STC-a ili za procjenu utjecaja STC-a na kronične upalne ili maligne bolesti.

Abstract (english)

The data on direct human exposure to the mycotoxin sterigmatocystin (STC) is limited, even though STC is highly prevalent in indoor environments. Thus, we decided to conduct an analysis of blood plasma from voluntary blood donors to assess the exposure of healthy individuals to this mycotoxin. The method for extracting STC from blood plasma samples (N = 94) followed the modified QuEChERS method, in which acidic acetonitrile extraction was combined with the addition of salts and purification through SPE columns. The prepared extracts were analyzed using the LC-MS/MS method. The analysis confirmed the presence of STC in 21 out of 94 samples, but only in trace amounts, allowing us to conclude that STC exposure does not pose a health risk to the studied population. In the future, this method could be used in assessing human and animal exposure to STC and evaluating its toxicokinetics. This would be particularly important for individuals residing in areas where increased concentrations of STC have been detected, or to evaluate implication of STC in chronic inflammatory or malignant diseases.

• Keywords: sterigmatocistin; krv; plazma; SPE; LC-MS/MS; STC
• Keywords (english): sterigmatocystin; blood; plasma; SPE; LC-MS/MS; STC
• Language: croatian
• URN:NBN: urn:nbn:hr:163:407640
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-25 13:37:17