Usporedba određivanja brzine sedimentacije eritrocita na automatskom analizatoru SRS 100/II i ručno modificiranom Westergren metodom

master's thesis

• Title (english): Comparison of erythrocyte sedimentation rate measurement on automated SRS 100/II analyser and manually by modified Westergren method
• Author: Helena Oroz
• Mentor: Marija Grdić Rajković (mentor)
• Mentor: Sandra Margetić (mentor)
• Committee member: Marija Grdić Rajković (predsjednik povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Committee member: Sandra Margetić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2024-07-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Brzina sedimentacije eritrocita rutinska je metoda koja se često koristi u medicinsko-biokemijskim
laboratorijima. Westergren metoda referentna je metoda, no danas se često u upotrebi nalaze
automatizirane i modificirane metode za određivanje brzine sedimentacije. Svrha ovog ispitivanja
usporedba je automatizirane modificirane metode na SRS 100/II analizatoru (Greiner Bio-One,
GmbH, Austrija) i ručne modificirane metode na graduiranom stalku. Budući da analiza na SRS
100/II analizatoru i... More na graduiranom stalku iziskuje nove spremnike, prije usporedbe metoda
provedena je tehnička i klinička validacija spremnika VACUETTE® Tube 4NC ESR volumena 1,5
i 2,75 mL s 3,2%-tnim natrijevim citratom. Također, ispitana je i ponovljivost pomoću uzoraka,
jednog unutar, jednog iznad referentnog intervala. Utvrđena je primjerenost uvođenja obaju
spremnika u rutinski rad, no, s obzirom na to da analizator koristi samo spremnike volumena 1,5
mL, usporedivost je ispitana samo na njima. SRS 100/II posjeduje mogućnost mjerenja u trajanju od
15 i 30 minuta, tako da su uspoređivani rezultati dobiveni i nakon 15 minuta i nakon 30 minuta s
rezultatima dobivenim na graduiranom stalku nakon 30 minuta. Statističkom analizom utvrđeno je
da za mjerenje u trajanju od 30 minuta ne postoji klinički značajno konstantno odstupanje, dok za
mjerenje u trajanju od 15 minuta da postoji klinički značajno konstantno odstupanje, stoga je
prihvaćena metoda na SRS 100/II s mjerenjem u trajanju od 30 minuta za uvođenje u rutinski rad.
Ispitivanjem ponovljivosti na toj metodi, dobiveni rezultati zadovoljavaju kriterije prihvatljivosti. Less

Abstract (english)

Erythrocyte sedimentation rate is a routine method commonly used in medical-biochemical
laboratories. While the Westergren method is a reference method, today automated and modified
methods for measuring the sedimentation rate of erythrocytes are often in use. The purpose of this
examination is to compare the automated modified method on SRS 100/II analyzer (Greiner BioOne, GmbH, Austria) and the manual modified method on the graduated stand. Since the analysis
on the SRS 100/II analyser... More and on the graduated stand requires new test tubes, a technical and
clinical validation of THE VACUETTE ® tube 4NC ESR test tubes of 1.5 and 2.75 ml with 3.2%
sodium citrate was performed prior to the comparison of the methods. Also, repeatability using
samples was tested. The appropriateness of introducing both test tubes into routine was established,
however, since the analyser only uses 1,5 ml test tubes, comparability was only tested on them. The
SRS 100/II has the possibility of measuring for 15 and 30 minutes, so the results obtained after both
15 minutes and 30 minutes were compared with the results obtained on the graduated stand after 30
minutes. A statistical analysis showed that there is no clinically significant constant difference for a
30 minute measurement, while a clinically significant constant difference was found for a 15 minute
measurement. Therefore, a 30 minute measurement SRS 100/II method was adopted for routine
implementation. By testing repeatability on this method, the results were found to meet the
acceptability criteria. Less

• Keywords: sedimentacija eritrocita; brzina sedimentacije eritrocita; ručna modificirana metoda; automatizirana modificirana metoda; SRS 100/II
• Keywords (english): erythrocyte sedimentation; erythrocyte sedimentation rate; manual modified method; automated modified method; SRS 100/II
• Language: croatian
• URN:NBN: urn:nbn:hr:163:029839
• Study programme: Title: Medical biochemistry - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-26 11:10:18