Određivanje metala u dodacima prehrani za liječenje upalnih bolesti crijeva grafitnom tehnikom

master's thesis

• Title (english): Determination of metals in dietary supplements used for the treatment of inflammatory bowel diseases using graphite furnace technique
• Author: Jona Petić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Toma Keser (član povjerenstva)
• Committee member: Branimir Plavša (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2024-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Pojavnost upalnih bolesti crijeva je sve učestalija u posljednjih nekoliko desetljeća širom svijeta, a
samim time raste i potražnja i potrošnja dodataka prehrani koji imaju blagotvorni učinak na
liječenje bolesti. Bilo da se koriste kao suportivna terapija ili primarna terapija iznimno je važno da
su takvi pripravci dobro kontrolirani i da je poznat njihov kompletan sastav kako bi se osigurala
sigurnost korisnika. Metali se dijele na esencijalne i neesencijalne. Esencijalni su neophodni za
život ljudi u dobro definiranim količinama. Svi metali su toksični u većim dozama i mogu izazvati
kronične metaboličke poremećaje u organizmu. Upravo iz tog razloga nastoji se što bolje
kontrolirati njihov sadržaj u lijekovima, dodacima prehrani i hrani. Cilj rada bio je analizirati
dodatke prehrani koji se koriste za liječenje upalnih bolesti crijeva. Analizirani dodaci prehrani
sadrže crni papar, indijski tamjanovac, justiciju i/ili kurkumu kao biljne droge, a prikupljeni su u
trgovinama zdravom hranom ili putem internet trgovine. Za određivanje sadržaja kroma,
molibdena i kositra korištena je grafitna atomska apsorpcijska spektrometrija nakon uklanjanja
složene matrice spaljivanjem uzoraka mikrovalnom digestijom. Sadržaj metala u svim
analiziranim uzorcima bio je ispod propisanih maksimalnih vrijednosti propisanim od strane
regulatornih tijela.

Abstract (english)

The prevalence of inflammatory bowel diseases has been increasing globally over the past few
decades, leading to a growing demand and consumption of dietary supplements with beneficial
effects on disease treatment. Whether used as supportive or primary therapy, it is crucial that such
preparations are well-regulated and their complete composition is known to ensure user safety.
Metals are categorized into essential and non-essential. Essential metals are necessary for human
life in well-defined quantities. All metals are toxic in larger doses and can cause chronic metabolic
disorders in the body. Therefore, the content of drugs, dietary supplements, and food is strictly
controlled. The aim of this study was to analyze dietary supplements used for treating
inflammatory bowel diseases. The analyzed supplements contain black pepper, Indian
frankincense, justice, and/or turmeric as herbal drugs, and were collected from health food stores
or through online shops. Graphite atomic absorption spectrometry was used after removing the
complex matrix by microwave digestion of the samples to determine the content of chromium,
molybdenum, and tin. The metal content in all analyzed samples was below the maximum values
prescribed by regulatory bodies.

• Keywords: krom; molibden; kositar; dodaci prehrani; upalne bolesti crijeva; GFAAS
• Keywords (english): chromium; molybdenum; tin; dietary supplements; inflammatory bowel diseases; GFAAS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:665048
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-07-16
• Created on: 2024-09-27 11:20:59