Slobodna cirkulirajuća DNA u osteoartritisu

master's thesis

• Title (english): Circulating free DNA in osteoarthritis
• Author: Klara Ćosić
• Mentor: Sandra Šupraha Goreta (mentor)
• Committee member: Sandra Šupraha-Goreta (predsjednik povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Committee member: Najda Rudman (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2024-08-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Osteoartritis (OA), kronična i degenerativna bolest zglobova, najčešći je oblik artritisa. Često se zanemaruje ozbiljnost ove raširene i kronične bolesti. Razvoj lijeka za modificiranje OA otežan je nedostatkom topivih biomarkera za rano otkrivanje OA. Cilj istraživanja OA biomarkera je identificirati rani OA prije pojave radiografskih znakova i razvoja boli. Slobodna cirkulirajuća DNA (cfDNA) mogla bi poslužiti kao neivazivni biomarker OA. Uspoređene su dvije različite metode izolacije cfDNA i cilj je bio potvrditi koju je od njih bolje koristiti u budućoj genomskoj analizi s obzirom na bolji prinos ili čistoću. Istraživanje izvanstaničnog genomskog materijala također može otkriti više o temeljnoj patofiziologiji OA, stoga postoji potreba za definiranjem biomarkera za otkrivanje i praćenje napredovanja OA bolesti. Minimalno invazivne metode tekuće biopsije kao što su uzorkovanje sinovijalne tekućine i krvi za uzimanje genomskog materijala mogu biti osjetljivije od radiografije u otkrivanju, dijagnozi i praćenju OA u budućnosti.

Abstract (english)

Osteoarthritis (OA), a chronic, debilitating and degenerative disease of the joints, is the most common form of arthritis. The seriousness of this prevalent and chronic disease is often overlooked. Disease modifying OA drug development is hindered by the lack of soluble biomarkers to detect OA early. The objective of OA biomarker research is to identify early OA prior to the appearance of radiographic signs and the development of pain. So, the aim of work was extracellular genomic material that could serve as biomarkers of OA, cell-free DNA (cfDNA). Two different cfDNA isolation methods were compared and the aim was to confirm which one is better to use in future genomic analysis considering better yield or purity. There is an unmet need for soluble biomarkers for detecting and then monitoring OA disease progression. Extracellular genomic material research may also reveal more about the underlying pathophysiology of OA. Minimally invasive liquid biopsies such as synovial fluid and blood sampling of genomic material may be more sensitive over radiography in the detection, diagnosis and monitoring of OA in the future.

• Keywords: slobodna cirkulirajuća DNA; cfDNA; osteoartritis; izolacija DNA
• Keywords (english): circulating free DNA; cfDNA; osteoarthritis; DNA isolation
• Language: croatian
• URN:NBN: urn:nbn:hr:163:005480
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-11 11:35:53