Živa u dodacima prehrani za liječenje upalnih bolesti crijeva

master's thesis

• Title (english): Mercury in dietary supplements for the treatment of inflammatory bowel diseases
• Author: Nina Ribarić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Ana Mornar Turk (član povjerenstva)
• Committee member: Najda Rudman (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2024-09-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Dodaci prehrani namijenjeni za pomoćno liječenje upalnih bolesti crijeva mogu imati značajnu
ulogu u liječenju tih bolesti zbog prisustva raznih biološki aktivnih. Također sve se češće koriste za
povećanje unosa esencijalnih vitamina i minerala, no zbog manje restriktivnih zakonskih zahtjeva i
nedovoljne kontrole, postoji rizik od kontaminacije i nuspojava. Cilj ovoga rada bio je procijeniti
kvalitetu dodataka prehrani mjerenjem koncentracije žive u tim pripravcima. Živa i njezini spojevi
spadaju među najtoksičnije okolišne kontaminante. Izloženost čak i malim količinama žive može
izazvati ozbiljne zdravstvene probleme, uključujući mutagene i teratogene učinke, oksidativni stres,
disfunkciju mitohondrija, te toksičnost za središnji i periferni živčani sustav, kao i bubrege. Upravo
zbog ovih razloga potrebno je odrediti moguće kontaminacije živom. Koncentracija žive u ispitanim
dodacima prehrani određena je atomskom apsorpcijskom spektrometrijom uz pomoć analizatora
žive. Ova metoda je korištena zbog njezine brzine i jednostavnosti. Rezultati istraživanja uspoređeni
su sa maksimalnom dopuštenom razinom žive u dodacima prehrani koja je postavljena od strane
različitih regulatornih tijela. Svi analizirani dodaci prehrani zadovoljavaju zahtjeve to jest razina
žive je ispod maksimalne dopuštene razine, osim u dva uzorka justicije. Odnosno, analizom rezultata
utvrđeno je da izračunate vrijednosti hipotetskog unosa žive u slučaju tjedne uporabe testiranih
pripravaka ne prelaze PTWI vrijednost (privremeni podnošljivi tjedni unos), ali primjenom dva
uzorka utvrđene su PDE vrijednosti iznad dozvoljenih. U zaključku ovog rada istaknuta je važnost
stalne kontrole sadržaja žive u tržišno dostupnim dodacima prehrani.

Abstract (english)

Dietary supplements intended for adjunct treatment of inflammatory bowel diseases can play a
significant role in treating these conditions due to the presence of various biologically active
substances. They are also increasingly used to boost the intake of essential vitamins and minerals.
Still, due to less stringent regulatory requirements and insufficient control, there is a risk of
contamination and side effects. This study aimed to assess the quality of dietary supplements by
measuring the mercury concentration in these products. Mercury and its compounds are among the
most toxic environmental contaminants. Exposure to even small amounts of mercury can cause
serious health problems, including mutagenic and teratogenic effects, oxidative stress, mitochondrial
dysfunction, and toxicity to the central and peripheral nervous systems, as well as the kidneys. For
these reasons, it is necessary to determine potential mercury contamination. The mercury
concentration in the tested dietary supplements was determined using atomic absorption
spectrometry with a mercury analyzer. This method was chosen for its speed and simplicity. The
research results were compared with the maximum allowable mercury level in dietary supplements
set by various regulatory bodies. All analyzed dietary supplements meet the requirements, meaning
the mercury levels are below the maximum permissible level, except in two justice samples. The
analysis of the results also showed that the calculated values of hypothetical mercury intake in the
case of weekly use of the tested products do not exceed the PTWI (provisional tolerable weekly
intake). Still, after orally administering two samples, the PDE values were determined to be above
the permitted ones. In the conclusion of this work, the importance of constant control of the mercury
content in commercially available food supplements is emphasized.

• Keywords: upalne bolesti crijeva; dodaci prehrani; živa; analizator žive
• Keywords (english): inflammatory bowel diseases; dietary supplements; mercury; mercury analyzer
• Language: croatian
• URN:NBN: urn:nbn:hr:163:580405
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-09-02
• Created on: 2024-10-15 11:24:41