Evaluacija metode za pripremu citoloških preparata likvora

master's thesis

• Title (english): Evaluation of the method for the preparation of cerebrospinal fluid cytological preparations
• Author: Tanja Brežni
• Mentor: Željka Vogrinc (mentor)
• Committee member: Željka Vogrinc (predsjednik povjerenstva)
• Committee member: Ana Kozmar (član povjerenstva)
• Committee member: Marija Miloš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2024-10-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Analiza cerebrospinalne tekućine (likvora) od ključne je važnosti za dijagnostiku neuroloških bolesti. Citološka analiza likvora često predstavlja izazov zbog malog broja stanica i potrebe za očuvanjem njihove morfologije tijekom obrade. U radu su evaluirane tri metode obogaćivanja likvora (albuminom, pool-om seruma i pool-om likvora) kako bi se smanjio raspad stanica i poboljšala kvaliteta citoloških preparata. Analizirano je 35 uzoraka likvora dobivenih lumbalnom punkcijom i postoperativnom drenažom. Stanice su brojane ručno u Fuchs-Rosenthalovoj komori i automatizirano na Sysmex XN1000 BF analizatoru. Statistička analiza pokazala je visoku sličnost između metoda brojanja za većinu staničnih populacija, osim za polimorfonuklearne leukocite. Sysmex analizator pokazao je tendenciju podcjenjivanja broja stanica, osobito kod uzoraka s manjim brojem stanica. Metoda obogaćivanja likvora albuminom kod pripreme preparata pokazala se kao najučinkovitija, s najmanjim udjelom stanica u raspadu i najvećom konzistentnošću rezultata. Usporedbom rezultata ručnog i automatiziranog diferenciranja dokazane su statistički značajne razlike samo u udjelu neutrofilnih granulocita između Sysmexa i preparata obogaćenih pool-om seruma. Ovim radom dokazano je da metoda obogaćivanja albuminom osigurava najbolju očuvanost stanica, omogućujući dobivanje kvalitetnih citoloških preparata, što doprinosi pouzdanijoj dijagnostici i boljoj obradi uzoraka likvora.

Abstract (english)

The analysis of cerebrospinal fluid (CSF) is of key importance for the diagnosis of neurological diseases. Cytological analysis of cerebrospinal fluid is often a challenge due to the small number of cells and the need to preserve their morphology during processing. The paper evaluated three methods of CSF enrichment (albumin, serum pool, and CSF pool) in order to reduce cell decay and improve the quality of cytological preparations. 35 cerebrospinal fluid samples obtained by lumbar puncture and postoperative drainage were analyzed. Cells were counted manually in a Fuchs-Rosenthal chamber and automatically on a Sysmex XN1000 BF analyzer. Statistical analysis showed high similarity between counting methods for most cell populations, except for polymorphonuclear leukocytes. The Sysmex analyzer showed a tendency to underestimate the number of cells, especially in samples with a lower number of cells. The method of enriching the cerebrospinal fluid with albumin during the preparation of the preparation proved to be the most effective, with the lowest proportion of cells in disintegration and the highest consistency of results. By comparing the results of manual and automated differentiation, statistically significant differences were demonstrated only in the proportion of neutrophil granulocytes between Sysmex and preparations enriched with serum pool. This work proved that the albumin enrichment method ensures the best preservation of cells, enabling obtaining high-quality cytological preparations, which contributes to more reliable diagnostics and better processing of cerebrospinal fluid samples.

• Keywords: likvor; diferenciranje; citološki preparat
• Keywords (english): cerebrospinal fluid; differentiation; cytological preparation
• Language: croatian
• URN:NBN: urn:nbn:hr:163:865403
• Study programme: Title: Medical biochemistry - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-03 13:42:46