Određivanje sadržaja i sastava eteričnog ulja kore cimetovca

master's thesis

• Title (english): Determination of essential oil content and composition in cinnamon bark
• Author: Jelena Žulj
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Maja Friščić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2024-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Ljekarnička droga Cinnamomi cortex dobiva se od kore pravog, cejlonskog cimetovca (Cinnamomum verum J. Presl.) i
tradicionalni je biljni lijek za blage gastrointestinalne tegobe. S obzirom da je kora cimetovca jedan je od najstarijih i
često korištenih začina koji se zbog svog aromatičnog eteričnog ulja uvelike koristi u kulinarstvu i prehrambenoj
industriji, na tržištu nalazimo kore i nekoliko drugih vrsta cimetovaca. U okviru diplomskog rada provedena je
fitokemijska analiza u svrhu određivanja sadržaja i sastava eteričnog ulja u deset komercijalnih uzoraka kore cimetovca.
Metodom destilacije pomoću vodene pare utvrđeno je da uzorci cjelovite biljne droge sadrže od 16 do 30 mL eteričnog
ulja /kg, dok tržišni uzorci kore u prahu sadrže značajno manje eteričnog ulja (7 do 10 mL/kg). Metodom plinske
kromatografije spregnute s tandemskom spektrometrijom masa (GC-MS/MS) određen je kemijski profil izoliranog
eteričnog ulja kore cimetovca. Identificirane su 51 do 61 sastavnica koje su sačinjavale od 94,76 % do 99,90 % eteričnog
ulja. Glavna sastavnica je trans-cimetni aldehid (56,96-89,86 %), dok su ostale manje zastupljene eugenol (0,96-8,03
%), cinamil acetat (0,20-5,09 %) i p-metoksi-cimetni aldehid (0,25-2,59 %). Kumarin koji može biti štetan po ljudsko
zdravlje utvrđen je u previsokoj koncentraciji u samo jednom od 10 analiziranih eteričnih ulja. Dobiveni rezultati
ukazuju na važnost identifikacije izvorišne biljne vrste i kontrole kakvoće biljnih droga i pripravaka koji se primjenjuju
u suvremenoj fitoterapiji.

Abstract (english)

Herbal drug Cinnamomi cortex consists of bark of the true Ceylon cinnamon tree (Cinnamomum verum J. Presl.) and is
a traditional herbal medicine for mild gastrointestinal complaints. Given that cinnamon bark is one of the oldest and
frequently used spices, which is widely used in the culinary and food industry due to its aromatic essential oil, we also
find barks of several other types of cinnamon on the market. For this graduate thesis, a phytochemical analysis was
carried out in order to determine the content and composition of essential oil in ten commercial samples of cinnamon
bark. Using the method of steam distillation, it was determined that the samples of the herbal drugs in toto contain from
16 to 30 mL of essential oil/kg, while the market samples of powdered bark contain significantly less essential oil (7 to
10 mL/kg). The chemical profile of the isolated cinnamon bark essential oil was determined by the method of gas
chromatography coupled with tandem mass spectrometry (GC-MS/MS). Between 51 and 61 components were
identified, which constituted from 94.76% to 99.90% of the essential oil. The main component is trans-cinnamaldehyde
(56.96-89.86%), while the other less abundant are eugenol (0.96-8.03%), cinnamyl acetate (0.20-5.09%) and p-methoxy
cinnamaldehyde (0.25-2.59%). Coumarin, which can be harmful to human health, was found in too high a concentration
in only one of the 10 analyzed essential oils. The obtained results indicate the importance of identification of the source
plant species and quality control of herbal drugs and preparations used in modern phytotherapy.

• Keywords: kora cimetovca; Cinnamomi cortex; cimetovo eterično ulje; GC-MS; cimetni aldehid; kumarin; fitoterapija
• Keywords (english): cinnamon bark; Cinnamomi cortex; cinnamon essential oil; GC-MS; cinnamaldehyde; coumarin; phytotherapy
• Language: croatian
• URN:NBN: urn:nbn:hr:163:580052
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-11-29
• Created on: 2025-02-05 14:26:15