Likvoreja: usporedba dvije metode kvalitativnog određivanja asijalotransferina

master's thesis

• Title: Likvoreja: usporedba dvije metode kvalitativnog određivanja asijalotransferina : usporedba dvije metode kvalitativnog određivanja asijalotransferina
• Title (english): Cerebrospinal fluid leak: comparison of two qualitative methods determining asialo-transferrin
• Author: Marko Iličković
• Mentor: Željka Vogrinc (mentor)
• Committee member: Željka Vogrinc (predsjednik povjerenstva)
• Committee member: Ana Kozmar (član povjerenstva)
• Committee member: Desiree Coen Herak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2024-10-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Likvoreja je stanje u kojem cerebrospinalna tekućina istječe kroz oštećenje u duri mater, vanjskoj
ovojnici koja štiti središnji živčani sustav. Likvoreja može imati ozbiljne posljedice, uključujući
infekcije poput meningitisa, te utječe na funkciju mozga zbog gubitka potpore. Glavni cilj rada bio
je usporediti dvije metode za kvalitativno određivanje asijalotransferina, biokemijskog biljega
likvora, u uzorcima sekreta. Asijalotransferin je specifičan za cerebrospinalnu tekućinu, a njegovo
kvalitativno određivanje pomaže u dijagnosticiranju likvoreje. U istraživanju su korištene in-house
metoda i komercijalni test proizvođača Sebia. In-house metoda pokazala se specifičnijom i točnijom,
ali Sebia metoda je brža i praktičnija. Obje metode pokazale su visoku osjetljivost (100%), dok je
in-house metoda imala bolju specifičnost i točnost. Istraživanje je također ukazalo na mogućnost
lažno pozitivnih rezultata zbog prisutnosti bakterija koje mogu interferirati s testom.
Zaključeno je da nema značajne razlike u osjetljivosti između dvije metode, ali je in-house metoda
preciznija. Autori naglašavaju važnost korištenja kliničke slike pacijenta za točnu interpretaciju
rezultata, kao i potrebu za slikovnim tehnikama kako bi se lokaliziralo mjesto istjecanja te omogućilo
pravovremeno liječenje.

Abstract (english)

Cerebrospinal fluid (CSF) leakage represents a condition where cerebrospinal fluid escapes through
a tear in the dura mater, the outer membrane that protects the central nervous system. CSF leakage
can have serious consequences, including infections like meningitis, and it can affect brain function
due to the loss of support. The main aim of the thesis was to compare two methods for the qualitative
determination of asialotransferrin, a biochemical marker of CSF, in fluid samples. Asialotransferrin
is specific to cerebrospinal fluid, and its detection aids in diagnosing CSF leakage. The study utilized
an in-house method and a commercial test from the manufacturer Sebia. The in-house method proved
to be more specific and accurate, while the Sebia method was faster and more practical. Both
methods demonstrated high sensitivity (100%), but the in-house method had better specificity and
accuracy. The research also highlighted the potential for false-positive results due to the presence of
bacteria that can interfere with the test.
It was concluded that there is no significant difference in sensitivity between the two methods,
though the in-house method is more precise. The authors emphasize the importance of using the
patient's clinical picture for accurate interpretation of results, as well as the need for imaging
techniques to localize the site of leakage and enable timely treatment.

• Keywords: likvoreja; asijalotransferin; nazolikvoreja; bakterijski meningitis; elektroforeza
• Keywords (english): CSF leak; asialo-transferrin; CSF rhinorrhea; bacterial meningitis; electrophoresis
• Language: croatian
• URN:NBN: urn:nbn:hr:163:442897
• Study programme: Title: Medical biochemistry - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-03-28 14:06:11