Usporedba N-glikanskih profila iz umbilikalne krvi novorođenčadi zdravih majki i majki koje boluju od dijabetesa

master's thesis

• Title (english): Comparison of the N-glycan profile of the umbilical cord blood of newborn children of healthy mothers and mothers who have diabetes
• Author: Rinea Barbir
• Mentor: Gordan Lauc (mentor)
• Committee member: Gordan Lauc (predsjednik povjerenstva)
• Committee member: Olga Gornik (član povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2016-05-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Pojam dijabetesa u trudnoći obuhvaća dvije vrste dijabetesa: pregestacijski dijabetes, koji podrazumijeva diabetes mellitus tip 1 (DMT1) i diabetes mellitus tip 2 (DMT2) te gestacijski dijabetes. Svaki od njih ukazuje na lošiju prognozu za majku i novorođenče, kako za vrijeme trudnoće tako i na samom porodu, ali i tijekom života. Promjene glikozilacije proteina koje se javljaju u dijabetesu tip 2 su već i prije istraživane i mogle bi se koristiti za probiranje osoba s predispozicijom za razvoj dijabetesa. Za analizu je sakupljeno 60 uzoraka plazmi dobivenih vađenjem umbilikalne krvi novorođenčadi koje su imale dijabetes tjekom trudnoće i majki koje nisu imale dijabetes za vrijeme trudnoće. Nakon pripreme uzoraka N-glikanski profil je analiziran korištenjem tekućinske kromatografije visoke djelotvornosti koja se zasniva na hidrofilnim interakcijama, a dobiveni rezultati su analizirani statističkim testovima. Iz dobivenih rezultata možemo zaključiti da bi gestacijski dijabetes za vrijeme trudnoće mogao uzrokovat porast koncentracije visoko razgranatih struktura i smanjenje većine biantenarnih struktura te porast biantenarnog disijaliniziranog glikana s račvajućim N-acetilglukozaminom kod novorođenčadi. Međutim, budući da nije postignuta statistička značajnost nakon korekcije na višestruko testiranje, istraživanje je potrbeno ponoviti na većem broju uzoraka da bi to sa sigurnošću mogli potvrditi.

Abstract (english)

The term diabetes in pregnancy comprises two types of diabetes: pregestacional diabetes, which means diabetes mellitus type 1 (DMT1), diabetes mellitus type 2 (DMT2) and gestational diabetes. Each of them indicates a poor prognosis for the mother and baby, both during pregnancy and at the birth, and throughout life. Changes in glycosylation of proteins that occur in diabetes type 2 are before explored and could be used to screen people with a predisposition to develop diabetes.For the analysis it has been collected 60 samples of plasma obtained by extracting the umbilical cord blood of newbornes of mother who had diabetes during pregnancy and mothers who had not had diabetes during pregnancy. After sample preparation, N-glycan profile was analyzed using high performance liquid chromatography, which is based on hydrophilic interactions, and given results were analyzed with statistical tests. From the results we can conclude that gestational diabetes during pregnancy could cause increased concentration of highly branched structures and reducing most of the biantennary structures and increase of biantennary glycan with two sialic acids with branching N-acetylglucosaminin in newbornes. However, since we did not got any statistical significance after the correction on multiple testing, research requires to be repeated on a larger number of samples in order to be able to state this with certainty.

• Keywords: dijabetes; trudnoća; glikozilacija; glikanski profil
• Keywords (english): diabetes; pregnancy; glycosilation; glycan profile
• Language: croatian
• URN:NBN: urn:nbn:hr:163:941762
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-12-01 12:26:13