Aktivnost salivarne alfa amilaze u ovisnosti o metodi uzorkovanja

master's thesis

• Title (english): Activity of salivary alpha amylase in dependance of the sampling method
• Author: Marija Radiček
• Mentor: Nada Vrkić (mentor)
• Committee member: Nada Vrkić (predsjednik povjerenstva)
• Committee member: Olga Gornik (član povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2016-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Slina je tjelesna tekućina složenog sastava. Izlučevina je iz velikih i malih žlijezda slinovnica, čijim lučenjem upravlja autonomni živčani sustav. Ukupna slina je karakterizirana pisutnošću enzima amilaze u velikim količinama. Zbog
svog sastava, ukupna slina je pogodna za cijeli niz dijagnostičkih postupaka. Činjenica da se u slini nalaze komponente plazme omogućuje njihovu detekciju i kvantifikaciju iz uzorka sline. Stres je stanje u kojem je poremećena unutrašnja (psihofiziološka) ravnoteža organizma i koje zahtjeva ulaganje dodatnih napora radi prilagodbe. Trenutno se u istraživanju stresa koristi nekoliko salivarnih biomarkera kao što su: koncentracije salivarnog kortizola, sAA, sIgA i dehidroepiandrosteron (DHEA). sAA se koristi kao marker stresa te je visoko osjetljiv u stresnim situacijama uključujući i psihički stres te fizički stres. Cilj je ovog rada izmjeriti u slini koncentracije α-amilaze biokemijskog pokazatelja stresa, u zdravoj populaciji. Nadalje, želimo provjeriti postoje li razlike u vrijednostima α-amilaze uzorkovane na pasivan način te na stimuliran pomoću Salivetta i postoji li razlika u slini koju uzimamo na pasivan način, a da je pacijentu prethodno uzorkovana slina stimuliranom metodom. Nije dobivena statistički značajna povezanost na utjecaj koncentracije sAA ako prije pasivne metode uzorkovanja provodimo stimuliranu, iako je zabilježen porast koncentracije salivarne sAA nakon stimuliranog uzorkovanja.

Abstract (english)

Saliva is bodily fluid of complex composition. Total saliva is characterised with presence of amylase in large quantities. Because of its composure, total saliva is acceptable for a lot of diagnostic methods. The fact that some components from plasma are found in saliva allows their detection and quantification from sample of saliva. Stress is a state of uneven internal (psycophisiological) balance of organism which requires extra measures for adjustment.Currently, in research of stress, several salivary biomarkers are used, like: concentration of salivary cotisole, sAA, sIgA and dehydroepiandrosterone (DHEA). sAA is used as stress marker, and is highly sensitive in stress situations which include psyhical stress and physical stress. Aim of this thesis is to measure salivary concentration of alpha amylase, biochemical stress marker, in healthy population. Also, we want to see the differences in values of alpha amylase in saliva sample taken by passive way, and taken by using Salivetta and to see if there is difference in taking sample of saliva passively, but patient had previosly given saliva sample by stimulation method. There is no statistical significant correlation on the impact on the concentration of salivary alpha amylase when we conduct stimulative method before passive however salivary alfa amylase was higher after stimulative sampling.

• Keywords: slina; salivarna alfa amilaza; stres
• Keywords (english): saliva; salivary alpha amylase; stress
• Language: croatian
• URN:NBN: urn:nbn:hr:163:185114
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-12-02 16:44:39