Povezanost prosječnog volumena limfocita i monocita s aktivnošću bolesti kod reumatoidnog artritisa

master's thesis

• Title (english): Connection of mean lymphocyte volume and mean monocyte volume with disease activity in rheumatoid arthritis
• Author: Sara Sekelj
• Mentor: Renata Zadro (mentor)
• Committee member: Renata Zadro (predsjednik povjerenstva)
• Committee member: Nada Vrkić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2016-10-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Reumatoidni artritis je kronična autoimuna bolest od koje boluje otprilike I% populacije.
Dijagnostika ove bolesti temelji se na brojnim metodama uključujući radiološke i laboratorijske pretrage te
opću kliničku sliku. Laboratorijska dijagnostika uključuje KKS, upalne parametre (brzina SE, CRP) te
imunološke pretrage na različita antitijela, npr. anti-CCP. Uz pretrage krvi, analizira se i sinovijalna
tekućina. Iako ukupan broj leukocita s podatcima o udjelu pojedinih vrsta leukocita daje vrijednu informaiju
o aktivnosti bolesti, tehnologija VCS pruža informaciju o svakoj pojedinačnoj stanici i stoga biokemičarima
i kliničarima omogućava novi uvid u patofiziologiju upalnog procesa. U ovom radu proučene su vrijednosti
prosječnog volumena limfocita i monocita u definiranju aktivnosti bolesti korištenjem VCS tehnologije
automatskog analizatora na ukupno 140 uzoraka bolesnika i 32 uzorka ispitanika u kontrolnoj skupini.
Analizom podataka dobivena je statistički značajna razlika između vrijednosti MCV Ly i MCV Mo
kontrolne skupine ispitanika i bolesnika s dijagnosticiranim reumatoidnim artritisom te dobra korelacija s
CRP-om i DAS28 kod aktivne bolesti. Također, dobivena je statistički značajna razlika u vrijednostima
MCV Ly i MCV Mo u podskupinama aktivnog reumatoidnog artritisa i remisije bolesti. Uz definiranje
aktivnosti bolesti dobivene su statistički značajno više vrijednosti MCV Ly i MCV Mo kod bolesnika na
terapiji inhibitorima TNF-a u odnosu na bolesnike na terapiji inhibitorima IL-6. Dobiveni rezultati
potvrđuju MCV Ly i MCV Mo kao potencijalne nove biljege aktivnosti bolesti i vrijedna su tema daljnjih
istraživanja u ranoj dijagnostici, praćenju aktivnosti bolesti i terapije u različitim upalnim bolestima
uključujući reumatoidni artritis.

Abstract (english)

Rheumatoid arthritis (RA) is a chronic autoimmune disease with a prevelance of around 1% in the
world. Characterized by joint swelling, joint tenderness, and synovial hyperplasia, inflammatory process
eventually leads to cartilage and bone destruction, severe disability and premature mortality. Differencial
diagnosis is based on multiple diagnostic methods, including laboratory tests, radiography or other imaging
techniques. Along with specific inflammatory markers such as CRP or ESR, laboratory tests include
specific antibodies (anti-CCP) for RA diagnostics. Although total WBC count with cell population data
gives a valuable information about disease activity, VCS technology gives information about each
individua) leukocyte cell population therefore giving biochemists a new insight to the pathology of
inflammatory process. In this masters thesis, the VCS parameters mean lymphocyte volume (MCV Ly) and
mean monocyte volume (MCV Mo) acquired from a Beckman Coulter DxH800 haematology analyzer were
investigated to determine which of them could indicate the activation of inflammatory process in RA. Total number of samples was 140 in the test group and 32 in control group. Out of 140 samples, 34 were patients
with active rheumatoid arthritis and 104 were in remission. The results are as follows: MCV Ly is
significantly increased in patients with RA compared with control group as well as in patients with active
RA compared with those i11 remission. MCV Mo is also sig11ificantly increased in patients RA
compared with control group as well as in patients with active RA compared with those in remission. There
is a positive correlation between MCV Ly a11d MCV Mo and CRP and DAS28 in active rheumatoid
arthritis. MCV Ly a11d MCV Mo are also significantly increased in patients on anti-TNF-a:
with patients on anti-IL-6 drugs. These results indicate that MCV Ly and MCV Mo may have potential for
use in RA, monitoring disease activity as well as efficacy of therapy.

• Keywords: reumatoidni artritis; autoimunost; upala; VCS tehnologija; prosječni volumen limfocita; prosječni volumen monocita
• Keywords (english): rheumatoid arthritis; autoimmunity; inflammation; VCS technology; mean monocyte volume; mean lymphocyte volume
• Language: croatian
• URN:NBN: urn:nbn:hr:163:606301
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-13 11:02:05