Hipoglikemijski učinak Betule pendule, Roth. (Betulaceae) na aktivnost piruvat kinaze u HepG2 stanicama u hiperglikemijskim uvjetima

master's thesis

• Title (english): Hypoglicemic effect of Betula pendula, Roth. Betulaceae on pyruvate kinase activity in HepG2 cells under hyperglycemic conditions
• Author: Iva Čunović
• Mentor: Roberta Petlevski (mentor)
• Committee member: Roberta Petlevski (predsjednik povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2017-03-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Šećerna bolest je najčešća metabolička bolest karakterizirana dugoročnom hiperglikemijom, a uzrokovana je nedostatkom, smanjenom sekrecijom i/ili nepravilnom funkcijom inzulina.
U šećernoj bolesti su aktivnosti enzima glikolize, metaboličkog puta razgradnje glukoze, smanjene zbog nedostatka glukoze u stanici.
Cilj ovog rada je bio ispitati hipoglikemijski učinak Betule pendule, Roth. (Betulaceae), odnosno, učinak na aktivnost piruvat kinaze (PK), enzima koji katalizira posljednji korak glikolize.
Aktivnost enzima izmjerena je u lizatu stanica. Istraživanje je pokazalo statistički značajno smanjenje aktivnosti enzima pirvat kinaze u lizatu HepG2 stanica tretiranih 20 mM glukozom u odnosu na kontrolnu skupinu. Tretiranje HepG2 stanica Betulom pendulom, Roth. (0.05, 0.1 i 0.5 mg/mL) i 20 mM glukozom statistički je značajno povećalo aktivnost enzima u odnosu na skupinu tretiranu samo 20 mM glukozom.

Abstract (english)

Diabetes mellitus is widely spread epidemic disease characterized by chronic hyperglycemia that results from the absence of insulin, decreased secretion and /or impaired function.
In diabetes mellitus, glycolytic enzymes show decreased activity because no glucose is available in a cell. The aim of this thesis is to investigate hypoglycemic effect of Betula pendula, Roth. (Betulaceae) on the activity of pyruvate kinase, the enzyme that catalyzes the final step of glycolysis. Enzyme activity was measured in cell lysates. Treatment of cells with 20 mM glucose significantly decreased pyruvate kinase activity compared to the control group. Treatment with 20 mM glucose and Betula pendula, Roth. significantly increased pyruvate kinase activity compared to the group treated only with 20 mM glucose.

• Keywords: Betula pendula; Roth.; Betulaceae; piruvat kinaza; HepG2 stanica; hiperglikemija
• Keywords (english): Betula pendula; Roth.; Betulaceae; pyruvate kinase; HepG2 cell; hyperglicemic conditions
• Language: croatian
• URN:NBN: urn:nbn:hr:163:960812
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-24 14:47:04