Selektivna elektroanalitička metoda za određivanje sulfasalazina

master's thesis

• Title (english): Selective electroanalytical method for determination of sulfasalazine
• Author: Iva Mitrović
• Mentor: Biljana Nigović (mentor)
• Committee member: Biljana Nigović (predsjednik povjerenstva)
• Committee member: Željan Maleš (član povjerenstva)
• Committee member: Branka Zorc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2017-05-31, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Sulfasalazin je antireumatik koji se koristi u liječenju, reumatoidnog artritisa, ankiloznog spondilitisa, juvenilnog artirtisa, Chronove bolesti i ulceroznog kolitisa. Djeluje protuupalno i antibakterijski.
U ovom radu cikličkom voltametrijom ispitivan je utjecaj modifikacije površine elektrode od staklastog ugljika na voltametrijski odgovor sulfasalazina koji nastaje uslijed redukcije azo veze. Ispitane su modifikacije površine elektrode s grafenom, ugljikovim nanocjevčicama, bizmutovim nanočesticama i nanosupsenzijom bizmutovih nanočestica i ugljikovih nanocjevčica. Najviši strujni vrh zabiljeţen je na površini modificiranoj kombinacijom nanomaterijala, bizmutovih nanočestica i ugljikovih nanocjevčica, pri potencijalu od -0.2 V.
Zatim, ispitana je selektivnost nove elektroanalitičke metode praćenjem utjecaja interferencija drugih molekula na strujni vrh redukcije sulfasalazina pravokutnovalnom voltametrijom. Ispitivane su interferencije anorganskih tvari poput KNO3, ZnSO4, Na2CO3, KH2PO4, CuSO4, MgSO4, NaCl i FeCl3 te organskih molekule poput glukoze, laktoze, folne kiseline, askorbinske kiseline, dopamina i limunske kiseline te interferencije lijekova mesalazina, olsalazina i sulfadiazina. Elektroanalitička metoda razvijena na modificiranoj površini elektrode sinergističkim djelovanjem bizmutovih nanočestica i ugljikovih nanocjevčica pokazala je izvrsnu selektivnost i potencijal za voltametrijsko odreĎivanje sulfalazina u biološkim tekućinama.

Abstract (english)

Sulfasalazine is a disease-modifying antirheumatic drug and is used in the treatment of rheumatoid arthritis, ankylosing spondylitis, juvenile arthritis, Chron's disease and ulcerative colitis. It has anti-inflammatory and antibacterial characteristics.
In this paper, effect of the modification of the surface of glassy carbon electrode on the voltammetric response of sulfasalazine due to reduction of the azo-link was studied by cyclic voltammetry. Studied modifications of the surface were electrode with graphen, carbon nanotubes, bismuth nanoparticles and nanocomposite of bismuth nanoparticles and carbon nanotubes. The highest current peak was recorded on the surface modified with combination of nanomaterials, bismuth nanoparticles and carbon nanotubes at potential of -0.2 V.
Then, the selectivity of the method was investigated by monitoring the influence of the interference of other molecules to the peak current of sulfasalazine reduction by square wave voltammetry. The investigated molecules were inorganic molecules such as KNO3, ZnSO4, Na2CO3, KH2PO4, CuSO4, MgSO4, NaCl and FeCl3, organic molecules such as glucose, lactose, folic acid, ascorbic acid, dopamine and citric acid, and interferences of drugs mesalazine, olsalazine and sulfadiazine. The electroanalytical method developed on the modified surface of electrode by the synergistics effect of bismuth nanoparticles and carbon nanotubes showed excellent selectivity and potential for voltammetric determination of sulfasalazine in biological fluids.

• Keywords: sulfasalazin; ciklička voltametrija; pravokutnovalna voltametrija; bizmut nanočestice; ugljikove nanocjevčice; modificirana elektroda
• Keywords (english): sulfasalazine; cyclic voltammetry; square wave voltammetry; bismuth nanoparticles; carbon nanotubes; modified electrode
• Language: croatian
• URN:NBN: urn:nbn:hr:163:490826
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-24 15:38:43