Usporedba metoda za određivanje aktivnosti aminotransferaza sa i bez dodatka piridoksal fosfata

master's thesis

• Title (english): Comparison of methods for the determination of activity aminotransferases with and without addition of pyridoxal phosphate
• Author: Anđelka Milakara
• Mentor: Nada Vrkić (mentor)
• Committee member: Nada Vrkić (predsjednik povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Committee member: Roberta Petlevski (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Uvođenje nove metode u rutinski rad laboratorija obavezno zahtijeva provedbu verifikacije kako bi se
ispitale analitičke značajke koje je utvrdio proizvođač. Metoda određivanja aktivnosti aminotransferaza s
dodatkom piridoksal-fosfata (PLP) uključuje postupak verifikacije (ponovljivost, međulaboratorijska
preciznost, mjerna nesigurnost), provjeru istinitosti i stabilnosti te usporedbu s metodom bez dodatka PLP.
PLP se primjenuje zbog onih ispitanika koji imaju deficit PLP da bi se izbjegla lažna prosudba o stvarnom
sadržaju enzima. Cilj rada bio je provesti istraživanje na skupini bolesnika kod kojih je predvidiv deficit
PLP te na skupini ispitanika kod kojih se ne očekuje deficit PLP. Korišteni su ostatni uzorci krvi preostali
nakon rutinske laboratorijske analize bolničkih pacijenata u laboratoriju Kliničkog zavoda za kemiju KBC
Sestre milosrdnice. Rezultati analitičke verifikacije pokazali su da metoda imaju zadovoljavajuću
ponovljivost, međupreciznost te mjernu nesigurnost dok za mjerenje stabilnosti nije ispunila kriterije
prihvatljivosti koje je naveo proizvođeč. Ispitivanjem istinitosti te obradom rezultata Passing-Bablok
regresijom i Bland-Altman analizom pokazano je značajno postojanje razlike mjerenja enzimske aktivnosti
između metoda u svim ispitivanim skupinama. Metoda s dodanim PLP mjeri češće više vrijednosti, ali i
niže u odnosu na metodu bez PLP. Stoga zaključujemo da rezultati mjerenja s dodatkom PLP nisu
usporedivi s metodom bez dodatka PLP te se rezultati ne bi smjeli jednakovrijedno izdavati. To je važan
podatak jer liječnicima onemogućuje longitudinalno praćenje nalaza u vremenu prelaska na drugu metodu.

Abstract (english)

The introduction of a new analytical analyzer into routine clinical biochemistry laboratories requires
verification in order to test the analyzer's analytical features as defined by the manufacturer. The method of
determining aminotransferase activity with the addition of pyridoxal phosphate (PLP) involves the
verification procedure (repeatability, interlaboratory precision, measurament uncertainty), trueness checkup,
stability and comparison with a method without the addition of PLP. PLP is used for those who have a
deficit of PLP and to avoid a false assessment of the actual enzyme content. The aim of the study was to
conduct a study on a group of patients with predictable deficit of PLP and on a group of subjects with no
PLP deficit expected. Remaining blood samples were used after routine laboratory analysis of hospital
patients at the Clinical Institute of Chemistry KBC Sestre milosrdnice. The results of the analytical
verification showed that the methods had satisfactory repeatability, interpersonality and measurement
uncertainty while their stability did not meet the eligibility criteria. By verifying the trueness and processing
the results of Passing-Bablok regression and Bland-Altman analysis, there was a significant difference in
measurement of enzyme activity between the methods in all the investigated groups. The PLP method
measures higher values of the enzyme activity but also lower than the method without PLP. Therefore, we
conclude that the results of the measurements with the addition of PLP are not comparable to the method
without the addition of PLP and the results should not be published alternately. This is important to doctors
because they won't be able to longitudinally track the findings at the time of transition to another method.

• Keywords: piridoksal-fosfat; aminotransferaze; Passing-Bablok regresija; Bland-Altman analiza
• Keywords (english): pyridoxal phosphate; aminotransferases; Passing and Bablok regression; Bland and Altman analysis
• Language: croatian
• URN:NBN: urn:nbn:hr:163:344227
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-29 16:35:04