Utjecaj ekstrakcijskog otapala na određivanje odabranih kortikosteroida u prirodnim dermatološkim proizvodima

master's thesis

• Title (english): Influence of extraction solvent in determination of selected corticosteroids in herbal dermatological products
• Author: Martina Matičević
• Mentor: Miranda Sertić (mentor)
• Committee member: Miranda Sertić (predsjednik povjerenstva)
• Committee member: Ana Mornar Turk (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2017-08-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Danas se sve više koriste prirodni proizvodi kao alternativa tradicionalno korištenim medicinskim preparatima uz pogrešnu
pretpostavku da zato što su prirodni su i sigurni. Mnogi takozvani prirodni proizvodi mogu biti krivotvorine u koje su dodane
aktivne farmaceutske supstance. Cilj našeg istraživanja je pronaći najprikladnije ekstrakcijsko otapalo koje daje najbolji
analitički prinos za dodane sintetske koritkosteroide u topičke pripravke prije određivanja sadržaja kromatografskom
metodom.
Četiri različita ekstrakcijska otapala su korištena za određivanje devet odabranih kortikosteroida. U tu svrhu, u HALMED-u
je ranije razvijana i validirana analitička kromatografska metoda za određivanje devet kortikosteroida. Rezultati pokazuju da
je 0,1% V/V octena kiselina u metanolu najprikladnija ekstrakcijska otopina za četiri komponente: alklometazon dipropionat,
klobetazol propionat, mometazon furoat i betametazon dipropionat. Metanol je dao najbolji analitički prinos za betametazon
dipropionat, mometazon furoat i hidrokortizon acetat. Acetonitril je najbolje ekstrakcijsko sredstvo za metilprednizolon
acetat, betametazon valerat i deksametazon, dok je etanol najprikladniji samo za triamcinolon acetonid. Na temelju dobivenih
rezultata možemo zaključiti da ako bi se odjednom htjelo ekstrahirati što više kortikosteroida analiziranih u ovom radu,
najbolji ekstrakcijski uvjeti bi bili postignuti korištenjem acetonitrila kao ekstrakcijskog sredstva. Iza acetonitrila, drugi izbor
za ekstrakciju više od pola spomenutih kortikosteroida bio bi metanol ili 0,1% V/V octena kiselina u metanolu. Rezultati
upućuju na to da dodatak octene kiseline mijenja vrstu komponente koja se ekstrahira te s time i efikasnost ekstrakcijskog
postupka.

Abstract (english)

Natural herbal products are being used more and more today instead of traditional pharmaceutical products. Unfortunately
many so-called natural products can be counterfeit by the addition of the active pharmaceutical ingredient. The aim of the
study was to find the most appropriate extraction solution for the best recovery of added synthetic corticosteroids in topical
preparations before assay determination by chromatographic method.
Four different extraction solutions were used and nine corticosteroids were determined. For that purpose a validated
analytical chromatographic method was used, previously developed and validated for assay determination of above
mentioned compounds.
Results show that 0.1 % V/V acetic acid in methanol is the most appropriate extraction solution for 4 compounds namely,
alclometazon dipropionate, clobetason propionate, mometason fuorate and bethametazon dipropionate. Methanol gave the
best recovery for bethametazon dipropionate, mometason fuorate and hydrocortison acetate. Acetonitrile was the best choice for methylprednisolon acetate, bethametazon valerate and dexamethason., while ethanol was the best extraction solution for only triamcinolon acetinide. It can be seen from all of the obtained results that the best extraction conditions, if one would like to extract most of the above mentioned corticosteroids, would be accomplished by using acetonitrile as the extraction solution. Following acetonitrile, second choice for good extraction of more than half of mentioned corticosteroids would be both methanol or 0,1 % V/V acetic acid in methanol. The results suggest that addition of acetic acid changes the type of compounds that are extracted and the efficiency of the extraction procedure.

• Keywords: kortikosteroidi; otapalo; ekstrakcija; dermatološki proizvodi
• Keywords (english): corticosteroids; solvents; extraction; dermatological products
• Language: croatian
• URN:NBN: urn:nbn:hr:163:458474
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-12-07 14:15:41