Određivanje željeza u hrvatskim maslinovim uljima primjenom GF-AAS tehnike

master's thesis

• Title (english): Determination of iron in Croatian olive oils using GF-AAS technique
• Author: Margareta Talanga
• Mentor: Ana Mornar Turk (mentor)
• Committee member: Ana Mornar Turk (predsjednik povjerenstva)
• Committee member: Daniela Amidžić Klarić (član povjerenstva)
• Committee member: Željan Maleš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Budući da elementi u tragovima imaju negativne učinke na oksidativnu stabilnost maslinovog ulja i ljudsko zdravlje,
cilj ovog rada bio je odrediti koncentraciju željeza, elementa važnog u procjeni kvalitete maslinovih ulja.
Analiza ovog elementa u tragovima u 35 uzoraka maslinovog ulja porijeklom iz Republike Hrvatske provedena je
metodom atomske apsorpcijske spektrometrije grafitnom tehnikom. Uzorci maslinovog ulja sadrže visoki udio lipidnih
sastavnica te predstavljaju složen organski sustav za određivanje metala. U cilju dobivanja homogene otopine prikladne
za određivanje metala grafitnom tehnikom svi analizirani uzorci prethodno su spaljeni mikrovalnom digestijom.
Provedenim istraživanjem uočene su prednosti određivanja koncentracije željeza grafitnom tehnikom nakon uklanjanja
složenog matriksa spaljivanjem uzoraka mikrovalnom digestijom. Niske RSD vrijednosti upućuju na zadovoljavajuću
ponovljivost i postupka mikrovalne digestije i određivanja koncentracije metala grafitnom tehnikom. Na temelju
dobivenih vrijednosti LOD i LOQ te definirane NDK, provedene metode grafitne tehnike mogu se smatrati prikladnim
za određivanje analiziranog metala u maslinovim uljima.
Koncentracije željeza u istraživanim maslinovim uljima bile su ispod najviše dozvoljene koncentracije definirane
važećim pravilnikom osim u jednom uzorku te upućuju na dodatno praćenje zastupljenosti ovog metala u hrvatskih
maslinovih ulja. Potrebna su daljnja istraživanja u okviru zastupljenosti drugih metala u maslinovom ulju.
Istraživana maslinova ulja kategorizirana su kao ekstra djevičansko maslinovo ulje, dok je manji broj uzoraka
klasificirano u skupinu djevičansko maslinovo ulje.

Abstract (english)

Since heavy metals and trace elements have negative effects on the oxidative stability of olive oil and human health, the
aim of this study was to determine the concentrations of iron, the element important for the assessment of olive oil
quality.
The analysis of this trace element, in 35 samples of olive oil, originating from the Republic of Croatia, were performed
by atomic absorption spectrometry using graphite technique. The samples of olive oil contain a high proportion of lipid
components, and thus represent a complex organic system for the determination of metal. In order to obtain a
homogeneous solution suitable for the determination of metal by graphite technique, all analyzed samples were
previously prepared using a microwave digestion system.
The obtained results have shown the advantages of iron concentration determination by graphite technique after removal
of complex matrix by burning samples by microwave-assisted digestion. Low RSD values point out the
exceptional/satisfactory repeatability of microwave digestion procedures and the metal concentration determination by
graphite technique. Based on the obtained LOD and LOQ values as well as the defined NDK, conducted graphite
technique methods can be considered as suitable to determine the analyzed metal in olive oils.
The concentrations of iron in the investigated olive oils were below their maximum permissible
concentrations as defined by the valid rulebook, except in one sample. The presented data point to the exceptional quality
of Croatian olive oils. Further research should be conducted to evaluate the presence of other metals in the investigated
olive oil samples.
The investigated olive oils are categorized as extra virgin olive oil, while a small number of samples are
classified into the group of virgin olive oil.

• Keywords: željezo; maslinovo ulje; onečišćenja; grafitna AAS; mikrovalna digestija
• Keywords (english): iron; olive oil; impurities; graphite furnace AAS; microvawe digestion
• Language: croatian
• URN:NBN: urn:nbn:hr:163:911274
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-26 15:21:24