Verruculogen production in airborne and clinical isolates of Aspergillus fumigatus Fres.

Scientific paper - Original scientific paper

• Title (croatian): Tvorba verukulogena u kliničkih i iz zraka izoliranih sojeva vrste Aspergillus fumigatus Fres.
• Author: Ivan Kosalec
• Author: Maja Šegvić Klarić
• Author: Stjepan Pepeljnjak
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

Among airborne aspergilli sampled in outdoor air of the Zagreb area (2002/2003), Aspergillus niger (v. Teigh.) and A. fumigatus (Fres.) were the most abundant species (20-30%), with low mean annual concentrations (0.21-1.04 CFU m-3). Higher concentrations of A. fumigatus were observed in autumn and winter (0.5-1.05 CFU m-3) than in spring and summer (0-0.4 CFU m-3). On the other hand, A. fumigatus was found to be the most frequent isolate from upper and/or lower respiratory tracts of imunocompromised patients in many studies. This species produces several mycotoxins including the tremorgenic mycotoxin verruculogen that can be found in spores and during myceliar growth. Verruculogen production ability was tested by biosynthesis on 30 airborne and 33 clinical isolates of A. fumigatus. In both groups, high percentage of verruculogen-producing strains was noticed (84% of airborne and 91% of clinical isolates). Verruculogen production was not significantly different in the groups of airborne isolates (0.34 ± 0.16 mg mL-1) and clinical isolates (0.26 ± 0.19 mg mL-1, p > 0.05).

Abstract (croatian)

Uzorkovanje spora plijesni na području grada Zagreba obavljeno je tijekom godine 2002./2003. Pri tome su Aspergillus niger (v. Teigh.) i A. fumigatus (Fres.) bile najučestalije (20–30%) među aerogenim Aspergillus vrstama. Srednje godišnje koncentracije njihovih spora bile su izrazito niske (0,21–1,04 CFU m–3). Veće koncentracije spora A. fumigatus zabilježene su tijekom jeseni i zime (0,50–1,05 CFU m–3) u odnosu na koncentracije izmjerene tijekom proljeća i ljeta (0–0,40 CFU m–3). S druge strane, mnogobrojne studije ukazuju na visoku učestalost A. fumigatus izolata iz gornjih i donjih dišnih puteva imunokompromitiranih bolesnika. Ova plijesan tvori nekoliko različitih mikotoksina, uključujući i tremorgeni mikotoksin verukulogen koji se može naći u sporama i miceliju vrste A. fumigatus. Tvorba verukulogena ispitana je u 30 aerogenih i 33 klinička izolata A. fumigatus vrste. U obje skupine dokazana je visoka učestalost verukulogen-pozitivnih sojeva (84% aerogenih i 91% kliničkih izolata). Potencijal tvorbe verukulogena u aerogenih (0.34 ± 0.16 mg mL–1) i kliničkih sojeva (0.26 ± 0.19 mg mL–1) nije se značajnije razlikovao.

• Keywords (english): verruculogen; Aspergillus fumigatus; toxicity; mycotoxin; yeast extract
• Keywords (croatian): verukulogen; Aspergillus fumigatus; toksičnost; mikotoksin; ekstrakt kvasca
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 55, no. 4, pp. 357-364
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:549496
• Publication: 2005-12-01
• Document URL: https://hrcak.srce.hr/18119
• Type of resource: Text
• Access conditions: Open access
• Created on: 2018-02-27 12:21:57