Ochratoxin A induces apoptotic and necrotic renal cell death

Scientific paper - Original scientific paper

• Title (croatian): Okratoksin A inducira apoptotičko i nekrotičko umiranje bubrežnih stanica
• Author: József Petrik
• Author: Ana Malić
• Author: Karmela Barišić
• Author: Lada Rumora
• Author: Tamás Kõszegi
• Author: Stjepan Pepeljnjak
• Author: Tihana Žanić-Grubišić
• Author: Ivana Čepelak
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (english)

MDCK, LLC-PK1 and RK 13 renal cells were exposed to different OTA concentrations (2.5 to 25 μg OTA/mL of medium) for 24 hours. 2.5 μg OTA/mL significantly reduced the number of living RK 13 cells to 69.4 %, LLC-PK1 cells to 34.5 % and MDCK cells to 27.5 %, as compared to untreated control cells. Effects of OTA on cell morphology were examined by haematoxylin/ eosin or phalloidin-FITC/propidium iodide staining. 12 %, 11 % and 8.2 % apoptotic cells were observed in MDCK, LLC-PK1 and RK 13 cells treated with 2.5 μg OTA/mL, respectively. The structure of actin cytoskeleton showed significant changes in OTA-treated cells. Release of LDH into the culture medium was more abundant in MDCK and LLC-PK1 cells. OTA induced apoptotic and/or necrotic cell death in a cell type- and concentration-dependent manner. The results suggest that MDCK and LLC-PK1 cells are more sensitive to OTA than RK 13 cells.

Abstract (croatian)

MDCK, LLC-PK1 i RK 13 bubrežne stanice tretirane su različitim koncentracijama OTA (od 2,5 do 25 μg OTA/mL medija) tijekom 24 sata. Koncentracije toksina od 2,5 μg/mL uzrokuju značajno smanjenje broja živih stanica u odnosu na netretirane kontrolne uzorke: kod RK 13 stanica broj je smanjen na 69,4 %, kod LLC-PK1 na 34,5 %, a kod MDCK na 27,5 %. Ispitan je učinak OTA na morfologiju stanica koje su bojane hematoksilinom/ eozinom odnosno faloidin-FITC/propidijevim jodidom. Kod MDCK, LLC-PK1 i RK 13 stanica tretiranih s 2,5 μg OTA/mL opaženo je 12 %, 11 % odnosno 8,2 % stanica u apoptozi. OTA je uzrokovao značajne promjene u strukturi aktinskoga citoskeleta u tretiranim stanicama. OTA je potaknuo istjecanje LDH iz stanica u okolni medij, što je osobito izraženo u MDCK i LLC-PK1 stanicama. Možemo zaključiti da način umiranja stanica (apoptoza ili nekroza) ovisi o vrsti samih stanica i o primijenjenoj koncentraciji toksina. Naši rezultati ukazuju da su MDCK i LLC-PK1 stanice osjetljivije na djelovanje OTA od RK 13 stanica.

• Keywords (english): ochratoxin A; apoptosis; necrosis; MDCK cells; LLC-PK1 cells; RK 13 cells
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 78, no. 3, pp. 447-453
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• URN:NBN: urn:nbn:hr:163:960659
• Publication: 2005-09-15
• Document URL: https://hrcak.srce.hr/56
• Type of resource: Text
• Access conditions: Open access
• Created on: 2018-05-11 10:07:39