Utjecaj gama zračenja na sastav mikobiote slikarskog platna

master's thesis

• Title (english): Gamma-irradiation effect on mycobiota composition of the painting canvas
• Author: Marijana Ivin
• Mentor: Maja Šegvić Klarić (mentor)
• Committee member: Maja Šegvić Klarić (predsjednik povjerenstva)
• Committee member: Erim Bešić (član povjerenstva)
• Committee member: Katarina Marušić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2018-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U ovom radu proučavan je utjecaj gama zračenja naspram plijesni, ubikvitarnih mikroorganizama te jednih od najčešćih kolonizatora i uzroka propadanja predmeta kulturne baštine. Prevencija kao i sprječavanje daljne degradacije ovih predmeta, s visokim stupnjem učinkovitosti i sigurnosti, može se provesti korištenjem gama zračenja. Uzorci platna korišteni u istraživanju podijeljeni u dvije skupine (uzorci ne podvrgnuti vlažnoj inkubaciji i uzorci podvrgnuti vlažnoj inkubaciji prethodno zračenju), izloženi su zračenju izvora u Laboratoriju za radijacijsku kemiju i dozimetriju (LRKD) na Institutu Ruđer Bošković (IRB) u Zagrebu. Primijenjene su doze zračenja od 2, 7, 20 i 50 kGy pri dvjema brzinama zračenja (0,1 i 9,8 Gy s-1), nakon čega je slijedilo praćenje oporavka plijesni 0., 7., 14. i 28. dan od zračenja. Najzastupljenijim kolonizatorima platna pokazale su se Penicillium spp., Cladosporium spp., Aspergillus spp. te bijela plijesan i kvasci. Učinkovitije smanjenje koncentracije plijesni kod obje skupine uzoraka pri svim dozama zračenja zabilježeno je primjenom veće brzine zračenja (9,8 Gy s-1). S obzirom na bolji antifungalni učinak, posebice kod uzoraka inkubiranih u vlažnim uvjetima te dostupnih podataka iz literature o neškodljivosti gama zračenja za celulozne materijale, apsorpcijska doza od 7 kGy pri brzini zračenja od 9,8 Gy s-1, smatra se najboljim izborom. Najrezistentnijima su se pokazale Cladosporium spp. za čiju je otpornost zadužen pigment melanin prisutan unutar micelija plijesni te bijele plijesni i kvasci čije pronalaženje uzroka otpornosti leži u korištenju molekulsko-bioloških metoda u budućim ispitivanjima.

Abstract (english)

In this thesis the effect of gamma radiation on mould was studied. Mould is an ubiquitous microorganism and one of the most common coloniser and cause of deterioration of objects of cultural heritage and arts. Highly successful and safe prevention of further degradation of aforementioned objects can be done using gamma radiation. Painting canvas samples used in this research were divided in two groups and irradiated in the Radiation chemistry and dosimetry laboratory at Ruđer Bošković Institute in Zagreb. Radiation doses of 2, 7, 20 and 50 kGy we used at 2 different speeds (0,1 i 9,8 Gy s-1 ) followed by monitoring of mould recovery at days 0., 7., 14. and 28. The most frequently found mould on canvases were those of genus Penicillium spp., Cladosporium spp., Aspergillus spp. and white mould and yeast. Higher radiation speed (9,8 Gy s-1) was shown to be more effective in reducing mould concentration in both groups and at all radiation doses. Absorption dose of 7 kGy at the speed of 9,8 Gy s-1 was found to be the best choice, based on literature sources on safety of gamma radiation on cellulose materials and better antifungal effect, observed especially in samples incubated at a higher humidity. Cladosporium spp., white mould and yeast exhibited the highest resistance to radiation. Cladosporium spp. resistance can be attributed to pigment melanin found in its mycelium. Further research using molecular biology methods is needed to elucidate the mechanisms of white mould and yeast resistance.

• Keywords: kulturna baština; gama zračenje; plijesni
• Keywords (english): cultural heritage; gamma irradiation; moulds
• Language: croatian
• URN:NBN: urn:nbn:hr:163:276335
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-03 13:46:14