Verifikacija metoda za određivanje koncentracije novih oralnih antikoagulansa: dabigatrana, rivaroksabana i apiksabana

master's thesis

• Title (english): Verification of methods for determination of concentration of newer oral anticoagulants: dabigatran, rivaroxaban and apixaban
• Author: Ana-Katarina Kralj
• Mentor: Nada Vrkić (mentor)
• Committee member: Nada Vrkić (predsjednik povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Committee member: Sandra Margetić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2018-03-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Novi oralni antikoagulansi (NOAK) su lijekovi koji se posljednjih nekoliko godina sve više koriste u prevenciji i liječenju venske i arterijske tromboze. Metode kvantitativnog određivanja koncentracije NOAK lijekova tek postaju predmetom uvođenja u specijalizirane laboratorije za pretrage hemostaze i za sada još uvijek nisu sastavni dio laboratorijske dijagnostike.
Cilj ovog rada bio je ispitati ključna analitička obilježja kvantitativnih metoda određivanja koncentracije NOAK lijekova na dva različita analitička sustava kako bi se procijenilo jesu li ispitivane metode prikladne za uvođenje u svakodnevni rad. Ispitivanja metoda za određivanje koncentracije dabigatrana, rivaroksabana i apiksabana na analitičkim sustavima BCSXP (Siemens, Njemačka) i Compact MAX (Stago, Francuska) pokazala su zadovoljavajuće i prihvatljive kriterije ponovljivosti, unutarlaboratorijske preciznosti i linearnosti metoda. Stoga se zaključuje da su metode prikladne za uvođenje u redovnu laboratorijsku praksu. Ispitivana preciznost pokazala se nižom kod niskih koncentracija lijekova, što se može objasniti manjom preciznošću i točnošću mjerenja pri koncentracijama oko graničnih koncentracija kvantifikacije u odnosu na veće koncentracije lijeka.

Abstract (english)

New oral anticoagulants (NOACs) are drugs that have been increasingly used for the prevention and treatment of arterial and venous thrombosis in recent years. The methods for quantitative determination of concentration of NOAC drugs are just being introduced into specialized hemostasis laboratories and are currently not in routine laboratory use.
The aim of this study was to examine the key analytical characteristics of the quantitative methods for determination of NOACs on two different analytical systems in order to determine their suitability for routine laboratory use. The methods for the determination of concentration of dabigatran, rivaroxaban and apixaban on analytical systems BCSXP (Siemens, Germany) and Compact MAX (Stago, France) have met the criteria of repeatability, intralaboratory precision and linearity. Therefore, it was concluded that the methods are suitable for routine laboratory use. The determined precision was found out to be lower in the range of low drug concentrations, which can be explained by worse precision and measurement accuracy in the range of concentrations around the limit of quantification compared to higher drug concentrations.

• Keywords: novi oralni antikoagulansi; NOAK lijekovi
• Keywords (english): new oral anticoagulants; NOAC drugs
• Language: croatian
• URN:NBN: urn:nbn:hr:163:915450
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-13 11:03:12