Antioksidativni potencijal i udio polifenola u napitcima na bazi rogača

master's thesis

• Title (english): Antioxidant potential and polyphenolic share in carob based beverages
• Author: Andrea Matak
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Committee member: Mario Jug (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2018-03-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Rogač (Ceratonia siliqua, Fabaceae) je višegodišnja zimzelena biljka koja u današnje vrijeme najvećim dijelom raste na području Sredozemlja. Zahvaljujući visokom udjelu polifenola, poznatih po svom jakom antioksidativnom učinku, plod rogača je danas tema brojnih znanstvenih istraživanja. Svrha ovog rada bila je istražiti utjecaj zamjene dijela kakaa rogačevim brašnom (onim bez sjemenki, kao i onim sa sjemenkama) u smjesi za pripremu napitka na udio polifenola i antioksidacijski potencijal. Udio ukupnih fenola određen je Folin-Ciocalteu metodom, a antioksidativna učinkovitost je ispitana FRAP odnosno TEAC metodom. Rezultati istraživanja pokazali su da kakaovac sadrži više polifenola nego plod rogača te ima višu antioksidativnu učinkovitost. Uzorci sa povećanim udjelom brašna rogača pokazali su niži antioksidacijski potencijal i udio polifenola, međutim oni uzorci sa najvišim udjelom brašna rogača zadržali su značajan udio polifenola i antioksidacijsku aktivnost. Uočena je značajna razlika u sadržaju polifenola između uzoraka koji sadrže brašno rogača sa sjemenkama u odnosu na uzorke sa brašnom bez sjemenki rogača. Naime, uzorci sa brašnom rogača iz kojeg su uklonjene sjemenke pokazali su se kao puno vredniji izvor polifenola. Vrsta zaslađivača koja je dodana u uzorke korištene u ispitivanju nije bitno utjecala na sadržaj polifenola i ukupnu antioksidacijsku aktivnost. Rogač je bogat izvor brojnih bioaktivnih produkata koji su u posljednje vrijeme pokazali širok potencijal u terapiji i prevenciji različitih bolesti, čime se opravdava uloga rogača kao sve zastupljenije namirnice u prehrani čovjeka.

Abstract (english)

Carob (Ceratonia siliqua, Fabaceae) is an evergreen plant that nowadays grows mostly in Mediterranean. Due to the high content of polyphenols, known by their strong antioxidant activity, carob fruit has become the subject of numerous scientific researches. The purpose of this thesis was to explore the effect of replacing a part of cocoa powder with carob flour (those without seeds as well as those with seeds) in a mixture for the preparation of a beverage, on the content of polyphenols and antioxidant potential. The total phenolic content was determined by Folin-Ciocalteu method and antioxidant effectiveness was examined by FRAP and TEAC method. Results of the research showed that cocoa contains more polyphenols than carob fruit and has higher antioxidant effect. Samples with higher share of carob flour showed lower antioxidant potential and lower polyphenolic content, but those samples with the highest amount of carob flour kept a significant share of polyphenols and antioxidant activity. There was a significant difference in polyphenolic content in samples that contain carob flour with seeds compared to those containing carob flour without seeds. Namely, samples containing deseeded carob flour are shown to be a more valuable source of polyphenols. The type of sweetener added to the samples used in the research did not have a significant influence on polyphenolic content and antioxidant activity. Carob is a source of numerous bioactive products which have recently shown wide potential in therapy and prevention of various diseases, thus justifying the increasing role of carob in human nutrition.

• Keywords: rogač; kakao; kakaovac; polifenoli; antioksidansi
• Keywords (english): carob; cocoa; cocoa tree; polyphenols; antioxidants
• Language: croatian
• URN:NBN: urn:nbn:hr:163:511227
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-17 11:19:35