master's thesis
Testing the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties

Ines Kanski (2015)
Sveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
Zavod za biokemiju i molekularnu biologiju
Metadata
TitleTesting the interaction of flavonoids with protein disulfide isomerase PDIA3 and the effects on protein biological properties
AuthorInes Kanski
Mentor(s)Olga Gornik (thesis advisor)
Abstract
U okviru ovog diplomskog rada istražena je interakcija između odabranih flavonoida (eupatorin, apigenin, apigenin-7-glukozid, kempferol, naringenin, 3-O-metilkvercetin, cijanidin, genistein, luteolin-7-glukozid) sa proteinom ERp57 koji spada u skupinu disulfid-izomeraza (PDI). Za procjenu vezanja flavonoida sa proteinom ERp57 korištena je fluorescencijska spektrofotometrija i učinak gašenja fluorescencije, dok je za ispitivanje utjecaja flavonoida na vezanje ERp57-DNA korištena migracijska elektroforeza (EMSA). Također je testiran utjecaj flavonoida na aktivnost i stabilnost proteina. Apigenin i eupatorin pokazali su najmanju konstantu disocijacije, odnosno najveći afinitet za ERp57 te ih to čini najboljim molekulama za vezanje na protein ERp57. Potvrđena je važnost hidroskilne skupine za interakciju flavonoida sa proteinom.
Keywordsflavonoids apigenin eupatorin ERp57 fluorescence quenching
Committee MembersOlga Gornik
Sandra Šupraha Goreta
Suzana Inić
GranterSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
Lower level organizational unitsZavod za biokemiju i molekularnu biologiju
PlaceZagreb
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Study programme typeuniversity
Study levelintegrated undergraduate and graduate
Study programmePharmacy
Academic title abbreviationmag. pharm.
Genremaster's thesis
Language English
Defense date2015-09-14
Parallel abstract (English)
In this thesis, interaction between a protein that belongs to the family of protein disulfide isomerases (PDI)- protein ERp57, and chosen flavonoids- eupatorin, apigenin, apigenin-7-glucoside, kaempferol, naringenin, 3-O-Methylquercetin, cyanidin, genistein and luteolin-7-glucoside was tested. Interaction between ERp57 and flavonoids was assessed using fluorescence quenching method, while electrophorectic mobility shift assay (EMSA) was used to evaluate flavonoid effect on ERp57-DNA interaction. Flavonoid influence on protein activity and protein stability was also tested. Apigenin and eupatorin showed the lowest dissociation constant and the highest binding affinity to ERp57, making them the best compounds to be used as selective inhibitors/modulators of ERp57 biological activities.
Parallel keywords (Croatian)flavonoidi apigenin eupatorin ERp57 gašenje fluorescencije
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:163:378527
CommitterPetra Gašparac