prikaz prve stranice dokumenta Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
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Scientific paper - Original scientific paper
Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
Croatica Chemica Acta, 81 (2008), 1; 219-222. urn:nbn:hr:163:618042
Pavlinić, Dinko; Džijan, Snježana; Stipoljev, Feodora; Wagner, Jasenka; Ćurić, Goran; Lauc, Gordan

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Pavlinić, D., Džijan, S., Stipoljev, F., Wagner, J., Ćurić, G. & Lauc, G. (2008). Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies. Croatica Chemica Acta, 81. (1), 219-222. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, Dinko, et al. "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies." Croatica Chemica Acta, vol. 81, no. 1, 2008, pp. 219-222. https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, Dinko, Snježana Džijan, Feodora Stipoljev, Jasenka Wagner, Goran Ćurić and Gordan Lauc. "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies." Croatica Chemica Acta 81, no. 1 (2008): 219-222. https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, D., et al. (2008) 'Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies', Croatica Chemica Acta, 81(1), pp. 219-222. Available at: https://urn.nsk.hr/urn:nbn:hr:163:618042 (Accessed 30 April 2024)

Pavlinić D, Džijan S, Stipoljev F, Wagner J, Ćurić G, Lauc G. Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies. Croatica Chemica Acta [Internet]. 2008 [cited 2024 April 30];81(1):219-222. Available at: https://urn.nsk.hr/urn:nbn:hr:163:618042

D. Pavlinić, S. Džijan, F. Stipoljev, J. Wagner, G. Ćurić and G. Lauc, "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies", Croatica Chemica Acta, vol. 81, no. 1, pp. 219-222, 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:618042. [Accessed: 30 April 2024]

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Metadata
Title (english)Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
AuthorDinko Pavlinić
AuthorSnježana Džijan
AuthorFeodora Stipoljev
AuthorJasenka Wagner
AuthorGoran Ćurić
AuthorGordan Lauc
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract (english)
Autosomal trisomies account for more than 80 % of significant chromosomal disorders and are routinely detected by the cytogenetic analysis of cultivated amniotic fluid cells. However, this approach is time-consuming and requires a significant level of training and expertise. The main aim of our work was to introduce QF-PCR to our lab, a quicker, simpler and cheaper method. We also aimed to evaluate the usefulness of the chosen marker set in the Croatian population and the reliability and accuracy of the obtained results. STR loci from chromosomes 13, 18 and 21 were co-amplified, separated by capillary electrophoresis and analysed. Characteristic triplets and/or 2:1 patterns were detected for trisomic samples while normal samples were either homozygous or heterozygous. The tested set of loci showed high heterozygosity and therefore a good potential for analyzing the Croatian population. The results of QF-PCR were in full compliance with the cytogenetic analysis which was also performed for cultivated amniotic fluid cell samples.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCroatica Chemica Acta
Numberingvol. 81, no. 1, pp. 219-222
p-ISSN0011-1643
e-ISSN1334-417X
URN:NBNurn:nbn:hr:163:618042
Publication2008
Document URLhttps://hrcak.srce.hr/23449
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-04-21 15:00:20