prikaz prve stranice dokumenta Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
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Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
Croatica Chemica Acta, 81 (2008), 1; 219-222. urn:nbn:hr:163:618042
Pavlinić, Dinko; Džijan, Snježana; Stipoljev, Feodora; Wagner, Jasenka; Ćurić, Goran; Lauc, Gordan

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Pavlinić, D., Džijan, S., Stipoljev, F., Wagner, J., Ćurić, G. i Lauc, G. (2008). Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies. Croatica Chemica Acta, 81. (1), 219-222. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, Dinko, et al. "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies." Croatica Chemica Acta, vol. 81, br. 1, 2008, str. 219-222. https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, Dinko, Snježana Džijan, Feodora Stipoljev, Jasenka Wagner, Goran Ćurić i Gordan Lauc. "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies." Croatica Chemica Acta 81, br. 1 (2008): 219-222. https://urn.nsk.hr/urn:nbn:hr:163:618042

Pavlinić, D., et al. (2008) 'Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies', Croatica Chemica Acta, 81(1), str. 219-222. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:163:618042 (Datum pristupa: 16.10.2024.)

Pavlinić D, Džijan S, Stipoljev F, Wagner J, Ćurić G, Lauc G. Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies. Croatica Chemica Acta [Internet]. 2008. [pristupljeno 16.10.2024.];81(1):219-222. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:163:618042

D. Pavlinić, S. Džijan, F. Stipoljev, J. Wagner, G. Ćurić i G. Lauc, "Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies", Croatica Chemica Acta, vol. 81, br. 1, str. 219-222, 2008. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:163:618042. [Citirano: 16.10.2024.]

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Naslov (engleski)Quantitative fluorescent PCR – a rapid approach to prenatal diagnostics of common autosomal aneuploidies
AutorDinko Pavlinić
AutorSnježana Džijan
AutorFeodora Stipoljev
AutorJasenka Wagner
AutorGoran Ćurić
AutorGordan Lauc
Autorova ustanovaSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
(Zavod za medicinsku biokemiju i hematologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Farmacija
Medicinska biokemija
Sažetak (engleski)
Autosomal trisomies account for more than 80 % of significant chromosomal disorders and are routinely detected by the cytogenetic analysis of cultivated amniotic fluid cells. However, this approach is time-consuming and requires a significant level of training and expertise. The main aim of our work was to introduce QF-PCR to our lab, a quicker, simpler and cheaper method. We also aimed to evaluate the usefulness of the chosen marker set in the Croatian population and the reliability and accuracy of the obtained results. STR loci from chromosomes 13, 18 and 21 were co-amplified, separated by capillary electrophoresis and analysed. Characteristic triplets and/or 2:1 patterns were detected for trisomic samples while normal samples were either homozygous or heterozygous. The tested set of loci showed high heterozygosity and therefore a good potential for analyzing the Croatian population. The results of QF-PCR were in full compliance with the cytogenetic analysis which was also performed for cultivated amniotic fluid cell samples.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatica Chemica Acta
Brojčani podacivol. 81, br. 1, str. 219-222
p-ISSN0011-1643
e-ISSN1334-417X
URN:NBNurn:nbn:hr:163:618042
Datum objave publikacije2008
URL dokumentahttps://hrcak.srce.hr/23449
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2020-04-21 15:00:20