Određivanje sadržaja polifenola u ekstra djevičanskom maslinovom ulju

master's thesis

• Title (english): Phenolic compound determination in extra virgin olive oil
• Author: Andrea Bošković
• Mentor: Cvijeta Jakobušić Brala (mentor)
• Committee member: Cvijeta Jakobušić Brala (predsjednik povjerenstva)
• Committee member: Ana Karković Marković (član povjerenstva)
• Committee member: Monika Barbarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of physical chemistry); Zagreb
• Defense date and country: 2019-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Maslinovo ulje bogato je polifenolnim spojevima s jakim antioksidacijskim učinkom. Istraživanja su pokazala da konzumacija ekstra djevičanskog maslinovog ulja pomaže u prevenciji brojnih bolesti modernog doba uzrokovanih stresnim i nezdravim načinom života. Upravo su polifenolni spojevi, zahvaljujući smanjenju štetnog djelovanja slobodnih radikala na lipide u tijelu, zaslužni za barem dio navedenih učinaka. U ovom diplomskom radu određen je sadržaj ukupnih fenolnih spojeva i o-difenola u uzorku maslinovog ulja, te je određena antioksidacijska aktivnost fenolnog ekstrakta ulja s DPPH testom. Za tu svrhu su izrađeni odgovarajući baždarni pravci, sa standardnom otopinom galne kiseline. Određen je sadržaj slobodnih masnih kiselina, te je utvrđeno da ispitana ulja pripadaju u kategoriju ekstra djevičanska. Rezultati analize pokazuju da je ispitano maslinovo ulje vrijedan izvor fenolnih spojeva sa znatnim udjelom posebno biomedicinski zanimljivih o-difenola, što je dodatno potvrđeno i s DPPH antioksidacijskim testom.

Abstract (english)

Olive oil is rich in polyphenolic compounds, that have been proven to show antioxidative activity. Numerous researches showed that consuming extra virgin olive oil helps with preventing many diseases associated with modern age unhealthy lifestyle. It's the antioxidants contained in it that are to bethanked for a solid portion of said effects. In this thesis, total phenolicando-diphenol content was determined in a nolive oil sample, as well as its phenolic extractantioxidative activity with DPPH method. For this purpose, calibrating lines were obtained, using standard gallicacid solution. Further more, free fatty acid content was determined and it was shown that the analyzed olive oil sample actually was an extra virgin olive oil sample. The results of this analysis show that the analyzed olive oil is rich in phenolic compunds, particularly in biomedically relevanto-diphenols. This was also confirmed with DPPH antioxidative test.

• Keywords: ekstra djevičansko maslinovo ulje; polifenoli; o-difenoli; antioksidansi; Folin-Ciocalteu reagens; DPPH; natrij molibdat
• Keywords (english): extra virgin olive oil; polyphenols; o-diphenols; antioxidants; Folin-Ciocalteu reagent; DPPH; sodium molybdate
• Language: croatian
• URN:NBN: urn:nbn:hr:163:813909
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-05-14 10:10:07