Primjena funkcionalnih ekstrakta iz prehrambenog otpada u sintezi nanočestica selena - utjecaj na stabilnost tijekom probave

master's thesis

• Title (english): Application of functional extracts from food waste in selenium nanoparticle synthesis - effect on stability during digestion
• Author: Karla Vitlov
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Mario Jug (član povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2021-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Selen je esencijalni element, često deficitaran u svakodnevnoj prehrani. Sve se češće preporučuje kao dodatak prehrani u ljekarničkoj praksi, najčešće u obliku selenita ili organskih oblika selena. Zbog potencijalno boljih farmakokinetičkih svojstava, intenzivno se istražuje mogućnost njegove per os primjene u obliku nanočestica elementarnog selena. U okviru ovog eksperimentalnog rada istražena je mogućnost zelene sinteze nanoselena korištenjem inkapsulacijskih sredstava izoliranih iz prehrambenog otpada te je ispitana njihova stabilnost i biodostupnost nakon simulirane gastrointestinalne digestije, a u odnosu na kemijski sintetizirane nanočestice. U tu svrhu korišteni su polifenolni ekstrakt komine masline te pročišćene i sirove frakcije pektina izoliranih iz kore mandarine i komine rajčice. Promjena koncentracija čestica nanoselena nakon postupka probave mjerena je primjenom UV-Vis spektrofotometrije. Nakon statističke obrade podataka zaključeno je kako odabir inkapsulacijskog sredstva bitno utječe na gastrointestinalnu stabilnost nanoselena. Nakon gastrične probave koncentracije nanočestica povećavaju se neovisno o korištenom inkapsulacijskom sredstvu. Nakon provedene intestinalne probave dolazi do većeg ili manjeg smanjenja koncentracije zelenih nanočestica ovisno o primijenjenom inkapsulacijskom sredstvu. Iako su kemijski sintetizirane nanočestice inkapsulirane polisorbatom pokazale najbolju gastrointestinalnu stabilnost, biodostupnost svih analiziranih nanočestica bila je zadovoljavajuća i kretala se u intervalu od 72,5% do 140,9%.

Abstract (english)

Selenium is an essential element often deficient in the daily diet. That is why selenium dietary supplements are increasingly recommended in pharmacy practices, most often in the selenite form or organic forms of selenium. Due to potentially better pharmacokinetics properties nano-forms of elemental selenium for oral administration are being extensively researched. In this work green synthesis of nano-selenium particles using food waste isolated encapsulating agents was investigated. The stability and bioavailability of obtained nanoparticles was tested after simulated gastrointestinal digestion and compared to chemically synthesised selenium nanoparticles. Polyphenolic extracts of olive pomace and raw and purified pectin fractions isolated from mandarin peel and tomato pomace were used as encapsulating agents. The concentration change of nano-selenium particles after simulated digestion was measured by UV-Vis spectrophotometry. After statistical analysis it was concluded that the type of the encapsulating agent has a great impact on the gastrointestinal stability of nano-selenium. After gastric digestion, a concentration increase of nanoparticles was noticed, regardless of the used encapsulating agent. After intestinal digestion, the concentration decrease of selenium nanoparticles was observed, and the significance depended on the encapsulating agent used. Although chemically synthesized nanoparticles encapsulated with polysorbate showed the best gastrointestinal stability, the bioavailability of all analyzed nanoparticles was satisfactory and ranged from 72.5% to 140.9%.

• Keywords: selen; nanoselen; nanočestice; inkapsulacijska sredstva
• Keywords (english): selenium; nano-selenium; nanoparticles; encapsulating agents
• Language: croatian
• URN:NBN: urn:nbn:hr:163:802388
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-09 13:22:35