Centrifugalna particijska kromatografija u izolaciji prirodnih produkata

master's thesis

• Title (english): Centrifugal partition chromatography in isolation of natural products
• Author: Tea Raos
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Ivana Perković (član povjerenstva)
• Committee member: Marijan Marijan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-06-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Centrifugalna particijska kromatografija (CPC) je novija preparativna tehnika koja se sve više uspješno koristi u izolaciji, pročišćavanju i ukoncentriravanju prirodnih produkata. U okviru ovog diplomskog rada prikazana su dosadašnja teorijska saznanja o tom tipu tekućinske kromatografiji bez čvrstog nosača te mogućnostima njezine primjene u području prirodnih produkata. Obzirom da su ekstrakti dobiveni iz biljnih sirovina kompleksne smjese, odjeljivanje njihovih bioaktivnih sastavnica složen je i dugotrajan proces. CPC metoda kombinira načela ekstrakcije i kromatografije i može se koristiti za izolaciju tvari sa širokim rasponom polarnosti. Značajka ovog sustava su tekuće stacionarne i mobilne faze pa se analiza i odjeljivanje sastavnica prirodnih produkata zasniva na raspodjeli otopljenih tvari između te dvije nemješljive tekuće faze. Najčešće korišten dvofazni sustav otapala koji se koristi u području prirodnih produkata je heksan/etil acetat/ metanol/ voda. Tehnika se pokazala kao učinkovita alternativa konvencionalnim preparativnim kromatografskim tehnikama, a karakterizira ju kraće vrijeme pročišćavanja i mogućnost injektiranja veće količine uzorka, kao i dobivanje veće količine spojeva visoke čistoće (> 90 %). Znanstvena istraživanja dokazuju uspješnost primjene u izolaciji i pročišćavanje različitih skupina prirodnih spojeva kao što su kanabinoidi, lignani, kumarini, antocijani, alkaloidi, flavonoidi, stilbeni, ksantoni, monoterpeni, seskviterpeni, saponini i različiti pigmenti. Obzirom na učinkovitost preparativnog pročišćavanja, ova bi vrsta kromatografije mogla zadovoljiti potražnju za velikim količinama čistih prirodnih spojeva potrebnim za daljnja biološka, farmakološka i klinička istraživanja odnosno za proizvodnju standarda za potrebe kemijskih analiza ili pak za dobivanje frakcioniranih i ukoncentriranih prirodnih produkata od interesa za farmaceutsku, prehrambenu i kozmetičku industriju.

Abstract (english)

Centrifugal partition chromatography (CPC) is a recent preparative technique that is increasingly used in isolation, purification and concentration of natural products. In this thesis, the theoretical finding about this type of liquid chromatography without a solid support and the possibilities of its application in the field of natural products are presented. Since extracts obtained from plant raw material are quite complex mixtures, the separation of their bioactive components is a complex process. The CPC method combines extraction and chromatography principles and it can be used to isolate substances with a wide range of polarity. The characteristics of this system are liquid stationary and mobile phases, so analysis and separation of the components of natural products are based on the distribution of solutes between two immiscible liquid phases. The most commonly used two phase solvent system for this purpose is hexane / ethyl acetate / methanol / water. The technique has proven to be an effective alternative to conventional preparative chromatographic techniques and it is characterized by a shorter purification time and possibility of injecting a larger quantity of samples, as well as by obtaining a higher quantity of high purity compounds (> 90 %). Scientific researches prove the success of application in the isolation and purification of various group of natural compounds such as cannabinoids, lignans, coumarins, anthocyanins, alkaloids, flavonoids, stilbenes, xanthones, monoterpens, sesquiterpens, saponins and various pigments. Given the efficiency of preparative purification, this type of chromatography could satisfy the demand for large quantities of pure natural compounds necessary for further biological, pharmacological and clinical research, of for the production of standards for the purposes of chemical analyses or for the production of fractionated and concentrated natural products of interest for pharmaceutical, food and cosmetic industry.

• Keywords: prirodni produkti; centrifugalna particijska kromatografija
• Keywords (english): natural products; centrifugal partition chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:163:825763
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-06-27
• Created on: 2022-07-14 15:33:18