Optimizacija metode za kvantifikaciju nikotina u duhanu plinskom kromatografijom sa spektrometrijom masa

master's thesis

• Title (english): Method optimization for quantification of nicotine in tobacco by gas chromatography-mass spectrometry
• Author: Marko Marić
• Mentor: Jasna Jablan (mentor)
• Mentor: Ivana Bačić (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Ivana Bačić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2022-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Duhan, Nicotiana tabacum, porodice Solanaceae, jedan je od najčešće konzumiranih sredstava ugode i ovisnosti,
najčešće pušenjem. Razlog njegove ovisnosti je nikotin, kao jedan od glavnih sastojaka samoga duhana. Ovisno
o vrstama duhana kao što su Virginia, Burley, Maryland ili Oriental, ovisit će i koncetracija nikotina u duhanu.
Nikotin djeluje na nikotinske acetilkolinske receptore (nAChR) stvarajući ovisnost, ali i brojne fiziološke te
patofiziološke učinke. Često ilegalno trgovanje duhanom, kao i njegova uzročnost brojnih požara, uz brojne
druge razloge, dovode do potrebe optimizacije metode kvantitativnog određivanja nikotina u duhanu. Tako je
metoda pripreme uzoraka duhana za kvantitativno određivanje nikotina u duhanu optimizirana Box-Behnkenovim
dizajnom temeljenim na metodi odzivne površine. Korištenjem tri varijable u spomenutom dizajnu, udjela
metanola u otapalu za ekstrakciju, vremena soniciranja te broja ekstrakcija, dobivene su optimalne vrijednosti
navedenih parametara za pripremu uzoraka duhana. Kvantitativno određivanje nikotina odrađeno je pomoću
plinske kromatografije sa spektrometrijom masa (GC-MS) koja je validirana provjerom brojnih validacijskih
parametara te zadovoljila postavljene kriterije prihvatljivosti. Razvijena metoda pokazala se prikladnom za
navedenu svrhu kvantitativnog određivanja nikotina u duhanu te će se moći koristiti u Centru za forenzična
ispitivanja, istraživanja i vještačenja „Ivan Vučetić'', MUP-a RH.

Abstract (english)

Tobacco, Nicotiana tabacum, Solanaceae, is one of the most widely used stimulants and addictive substances,
consumed mainly by smoking. The reason for its addictive nature lies in nicotine, one of the main components
of tobacco. Depending on the type of tobacco, such as Virginia, Burley, Maryland or Oriental, the nicotine
concentration in tobacco also varies. Nicotine is a supstrate of nicotine acetylcholine receptors (nAChR), which
are addictive and cause many other physiological and patophysiological effects. Illegal tobacco and its causality
in numerous fire incidents, among many other reasons, have led to the need to optimize the method of
quantitative analysis of nicotine in tobacco. The method of preparing tobacco samples for quantitative analysis
of nicotine in tobacco was optimized by Box-Behnken design based on response surface modeling (RSM). In
this design, three parameters were used, namely the percentage of methanol in the extraction solution, the
duration of sonication, and the number of extractions for which optimal results were obtained, which were then
used to prepare the tobacco samples. The quantitative analysis of nicotine in tobacco samples was performed
using Gas Chromatography-Mass Spectrometry which was validated by checking numerous validation
parameters that met the established acceptance criteria. The method was thus found to be suitable for the
quantitative analysis of nicotine in tobacco and will be used at the Ivan Vučetić Forensic Science Center in
Zagreb, Croatia.

• Keywords: duhan; nikotin; plinska kromatografija sa spektrometrijom masa; Box-Behnken; optimizacija metode
• Keywords (english): tobacco; nicotine; gas chromatography-mass spectrometry; Box-Behnken; method optimizaton
• Language: croatian
• URN:NBN: urn:nbn:hr:163:806539
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-07-22
• Created on: 2022-07-26 13:59:40